Quantification of transforming growth factor β1 (TGFβ1) mRNA expression in mouse preimplantation embryos and determination of TGFβ receptor (type I and type II) expression in mouse embryos and reproductive tract
Quantification of transforming growth factor β1 (TGFβ1) mRNA expression in mouse preimplantation embryos and determination of TGFβ receptor (type I and type II) expression in mouse embryos and reproductive tract
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DOI：
10.1093/molehr/7.11.1047
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发表时间：
2001-11-01
影响因子：
4
通讯作者：
Yeung, WSB
中科院分区：
文献类型：
--
作者：
Chow, JFC;Lee, KF;Yeung, WSB
我们假设转化生长因子β 1（TGF β 1）及其受体在植入前胚胎和生殖道之间的相互作用中发挥作用。为了研究这一假说，我们用RNA模拟物通过竞争性逆转录聚合酶链反应定量小鼠胚胎中TGF β 1 mRNA的表达。TGF β（1）首先在未受精的卵母细胞中检测到，在受精后消失，并在2-细胞期再次表达（4410 +/- 1330转录本/胚胎）。其表达逐渐增加，在8细胞期达到峰值（58 600 +/- 17 300个转录物/胚胎），并在桑椹胚期后迅速下降，在胚泡期达到1520 +/- 546个转录物/胚胎的浓度。8细胞期和桑椹胚期TGF β 1的mRNA水平显著高于其他细胞期（P < 0.05）。还研究了TGF受体在胚胎和生殖道中的表达。通过免疫组织化学方法检测I-细胞至囊胚期胚胎中TGF（1）I型（ALK-5）和II型TGF β受体。北方杂交和免疫组织化学显示，从妊娠第1天到第4天，输卵管中TGF β受体的表达恒定，而在子宫中，第3天TGF β I型受体的表达显著增加。在整个研究期间，子宫中TGF β II型受体的表达保持不变。这项研究表明，植入前小鼠胚胎产生TGF β（1），胚胎和生殖道在植入前阶段对TGF β（1）有反应。
We hypothesized that transforming growth factor beta1 (TGF beta (1)) and its receptors play a role in the interaction between the preimplantation embryo and the reproductive tract. To investigate this hypothesis, TGF beta (1) mRNA in mouse embryos was quantified by competitive reverse transcription-polymerase chain reaction using an RNA mimic. TGF beta (1) was first detected in the unfertilized oocyte, disappeared after fertilization and was expressed again at the 2-cell stage (4410 +/- 1330 transcripts/embryo). Its expression increased gradually, peaked at the 8-cell stage (58 600 +/- 17 300 transcripts/embryo) and declined rapidly after the morula stage reaching a concentration of 1520 +/- 546 transcripts/embryo at the blastocyst stage. The mRNA levels of TGF beta (1) at the 8-cell and morula stages were significantly higher than that at other cell stages (P < 0.05). The expression of TGF receptors in embryos and in the reproductive tract was also investigated. Both TGF(1) type I (ALK-5) and type II TGF beta receptors were detected in embryos from I-cell to blastocyst stage by immunohistochemistry. Northern hybridization and immunohistochemistry showed a constant expression of both TGF beta receptors in the oviduct from day 1 to day 4 of pregnancy, whilst in the uterus there was a marked increase in the expression of TGF beta type I receptor on day 3. Expression of TGF beta type II receptor in the uterus remained unaltered throughout the study period. This study has shown that preimplantation mouse embryos produce TGF beta (1) and that both the embryos and the reproductive tract are responsive to TGF beta (1) in the preimplantation period.