Human bone marrow stromal cell responses on electrospun silk fibroin mats
Human bone marrow stromal cell responses on electrospun silk fibroin mats
复制标题
DOI：
10.1016/s0142-9612(03)00609-4
复制
发表时间：
2004-03-01
期刊：
影响因子：
14
通讯作者：
Kaplan, DL
中科院分区：
文献类型：
--
作者：
Jin, HJ;Chen, JS;Kaplan, DL
具有纳米级直径的纤维由于生物材料支架的高表面积而提供益处。在这项研究中，静电纺丝丝素蛋白为基础的纤维，平均直径为700 +/- 50 nm的再生蚕丝水溶液制备。研究了人骨髓基质细胞（BMSCs）在蚕丝基质上的粘附、铺展和增殖。扫描电子显微镜（SEM）和MTT分析表明，静电纺丝基质支持BMSC附着和增殖超过14天的培养类似于天然丝素蛋白（类似于15 μ m的纤维直径）矩阵。静电纺丝基质在体外支持BMSC附着、扩散和生长的能力，结合丝蛋白基质的生物相容性和生物可降解性，表明这些生物材料基质作为组织工程支架的潜在用途。(C)2003爱思唯尔有限公司。保留所有权利。
Fibers with nanoscale diameters provide benefits due to high surface area for biomaterial scaffolds. In this study electrospun silk fibroin-based fibers with average diameter 700 +/- 50 nm were prepared from aqueous regenerated silkworm silk solutions. Adhesion, spreading and proliferation of human bone marrow stromal cells (BMSCs) on these silk matrices was studied. Scanning electron microscopy (SEM) and MTT analyses demonstrated that the electrospun silk matrices supported BMSC attachment and proliferation over 14 days in culture similar to native silk fibroin (similar to15 mum fiber diameter) matrices. The ability of electrospun silk matrices to support BMSC attachment, spreading and growth in vitro, combined with a biocompatibility and biodegradable properties of the silk protein matrix, suggest potential use of these biomaterial matrices as scaffolds for tissue engineering. (C) 2003 Elsevier Ltd. All rights reserved.