Immunohistochemical expression and colocalization of somatostatin, carboxypeptidase-E and prohormone convertases 1 and 2 in rat brain
Immunohistochemical expression and colocalization of somatostatin, carboxypeptidase-E and prohormone convertases 1 and 2 in rat brain
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DOI：
10.1016/j.neuroscience.2007.04.039
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发表时间：
2007-06-29
期刊：
影响因子：
3.3
通讯作者：
Kumar, U.
中科院分区：
文献类型：
--
作者：
Billova, S.;Galanopoulou, A. S.;Kumar, U.
许多肽在靶组织中成熟的加工取决于分选受体的存在。一些先前的研究预测羧肽酶-E（CPE）、激素原转化酶1（PC 1）和激素原转化酶2（PC 2）可能作为生长抑素（SST）的分选元件，使其成熟并加工成适当的靶标。然而，目前还不知道是否大脑，神经元培养，甚至内分泌细胞表达SST，CPE，PC 1和PC 2，并表现出共定位。因此，本研究采用过氧化物酶免疫组织化学、双标记间接免疫荧光免疫组织化学和Western blot分析，绘制了SST、CPE、PC 1和PC 2在大鼠不同脑区的分布图。此外，我们还确定了SST与CPE、PC 1和PC 2的共定位以及CPE与PC 1和PC 2的共定位。SST、CPE、PC 1和PC 2在大鼠脑内的分布具有明显的区域特异性。采用间接双标记免疫荧光法，我们观察到选择性神经元特异性共定位在皮层，纹状体和海马的区域特异性的方式。这些研究为SST、CPE、PC 1和PC 2之间以及CPE与PC 1和PC 2之间的共定位提供了第一个证据。皮层SST与CPE、PC 1和PC 2共定位于锥体和非锥体神经元。最重要的是，在纹状体和海马共定位主要是观察到选择性和优先在中间神经元。CPE还以区域特定的方式与PC 1和PC 2共定位。本实验结果为研究SST、CPE、PC 1和PC 2在大鼠脑内的分布和共定位提供了新的思路。综上所述，我们的数据预测CPE，PC 1和PC 2可能是SST成熟的潜在目标。(c)2007年IBRO。由爱思唯尔有限公司出版。保留所有权利。
The processing of many peptides for their maturation in target tissue depends upon the presence of sorting receptor. Several previous studies have predicted that carboxypeptidase-E (CPE), prohormone convertase 1 (PC1) and prohormone convertase 2 (PC2) may function as sorting elements for somatostatin (SST) for its maturation and processing to appropriate targets. However, nothing is currently known about whether brain, neuronal culture or even endocrine cells express SST, CPE, PC1 and PC2 and exhibit colocalization. Accordingly, in the present study using peroxidase immunohistochemistry, double-labeled indirect immunofluorescence immunohistochemistry and Western blot analysis, we mapped the distributional pattern of SST, CPE, PC1 and PC2 in different rat brain regions. Additionally, we also determined the colocalization of SST with CPE, PC1 and PC2 as well as colocalization of CPE with PC1 and PC2. The localization of SST, CPE, PC1 and PC2 reveals a distinct and region specific distribution pattern in the rat brain. Using an indirect double-label immunofluorescence method we observed selective neuron specific colocalization in a region specific manner in cortex, striatum and hippocampus. These studies provide the first evidence for colocalization between SST, CPE, PC1 and PC2 as well as CPE with PC1 and PC2. SST in cerebral cortex colocalized in pyramidal and non-pyramidal neurons with CPE, PC1 and PC2. Most importantly, in striatum and hippocampus colocalization was mostly observed selectively and preferentially in interneurons. CPE is also colocalized with PC1 and PC2 in a region specific manner. The data presented here provide a new insight into the distribution and colocalization of SST, CPE, PC1 and PC2 in rat brain. Taken together, our data anticipate the possibility that CPE, PC1 and PC2 might be potential target for the maturation of SST. (c) 2007 IBRO. Published by Elsevier Ltd. All rights reserved.