Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes
Reactivity of antibodies directed against human antigens with surface markers on canine leukocytes
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DOI：
10.1016/s0165-2427(96)05604-8
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发表时间：
1996-10-01
影响因子：
1.8
通讯作者：
Wojewodzka, U
中科院分区：
文献类型：
--
作者：
Galkowska, H;Waldemar, LO;Wojewodzka, U
使用一组抗人抗体来研究它们与犬白细胞的交叉反应性。通过免疫细胞化学染色在显微镜水平上进行标记。在 50 种抗体中，有 22 种与来自传入淋巴液和外周血的犬白细胞发生交叉反应。所有白细胞均与 MHM23 (CD18) 发生反应。两种抗 HLA DR 抗体 DK22 和 L243 主要与遮蔽细胞和 PHA 刺激的淋巴细胞发生反应，而 TAL1B5 与所有犬淋巴细胞发生交叉反应。用 ACT1、Ki-67 和 PC10 克隆产生的抗体在 PHA 刺激的淋巴细胞上鉴定激活标记 CD25、Ki-67、PCNA。犬嗜酸性粒细胞与 MHM6 (CD23) 抗体发生反应。大量抗体与犬淋巴覆盖细胞发生反应。仅在用神经氨酸酶处理细胞离心涂片后，犬粒细胞和淋巴细胞子集才用抗 CD15 抗体染色。
A panel of anti-human antibodies was used in order to investigate their cross-reactivity with canine leukocytes. The labeling was carried out at the microscopic level by immunocytochemical staining. Of 50 antibodies 22 cross-reacted with canine leukocytes from afferent lymph and peripheral blood. All leukocytes reacted with MHM23 (CD18). Two anti-HLA DR antibodies, DK22 and L243, reacted mostly with veiled cells and with PHA-stimulated lymphocytes, whereas TAL1B5 was cross-reactive with all canine lymphocytes. The activation markers CD25, Ki-67, PCNA were identified on PHA-stimulated lymphocytes with ACT1, Ki-67 and PC10 clone produced antibodies. Canine eosinophils reacted with MHM6 (CD23) antibody. A large number of antibodies reacted with canine lymph veiled cells. Canine granulocytes and a subset of lymphocytes were stained with the anti-CD15 antibody only after treatment of cytospins with neuraminidase.