Protein Coronas on Gold Nanorods Passivated with Amphiphilic Ligands Affect Cytotoxicity and Cellular Response to Penicillin/Streptomycin
Protein Coronas on Gold Nanorods Passivated with Amphiphilic Ligands Affect Cytotoxicity and Cellular Response to Penicillin/Streptomycin
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DOI：
10.1021/nn5002886
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发表时间：
2014-05-01
期刊：
影响因子：
17.1
通讯作者：
Hamad-Schifferli, Kimberly
中科院分区：
文献类型：
--
作者：
Kah, James Chen Yong;Grabinski, Christin;Hamad-Schifferli, Kimberly
我们探讨了四种不同类型的两亲性配体（ALs）如何影响金纳米棒（NRs）上蛋白质冠状结构的形成及其对细胞反应的影响。用十六烷基三甲基溴化铵包被的hit与聚氧乙烯[10]十六烷基醚、低聚茶胺和磷脂酰丝氨酸（PS）进行配体交换。从马血清（ES）中提取的蛋白冠状体在这些NR-ALs上形成，并检测其胶体稳定性、细胞摄取、增殖、氧化应激和基因表达。我们发现形成的蛋白冠及其胶体稳定性受到AL类型的影响，并且细胞对这些nr -AL-冠（NR-AL-ES）的反应既依赖于配体，也依赖于冠。我们还发现，普通细胞培养补充剂青霉素/链霉素的存在会影响NR-AL和NR-AL- es的胶体稳定性和细胞反应，这表明当纳米颗粒存在时，细胞反应不一定对笔/链球菌惰性。虽然蛋白质冠是细胞所看到的，但其底层的表面配体显然在形成和定义冠的物理特性方面起着重要作用，并最终影响细胞反应。此外，本研究的结果表明，细胞对NR-AL的行为不仅受AL的类型及其形成的蛋白冠的影响，还受其产生的胶体稳定性和与细胞培养补充剂的相互作用的影响。
We probe how amphiphilic ligands (ALs) of four different types affect the formation of protein coronas on gold nanorods (NRs) and their impact on cellular response. Hits coated with cetyltrimethylammonium bromide were ligand exchanged with polyoxyethylene[10]cetyl ether, oligofectamine, and phosphatidylserine (PS). Protein coronas from equine serum (ES) were formed on these NR-ALs, and their colloidal stability, as well as cell uptake, proliferation, oxidative stress, and gene expression, were examined. We find that the protein corona that forms and its colloidal stability are affected by AL type and that the cellular response to these NR-AL-coronas (NR-AL-ES) is both ligand and corona dependent. We also find that the presence of common cell culture supplement penicillin/streptomycin can impact the colloidal stability and cellular response of NR-AL and NR-AL-ES, showing that the cell response is not necessarily inert to pen/strep when in the presence of nanoparticles. Although the protein corona is what the cells see, the underlying surface ligands evidently play an important role in shaping and defining the physical characteristics of the corona, which ultimately impacts the cellular response. Further, the results of this study suggest that the cellular behavior toward NR-AL is mediated by not only the type of AL and the protein corona it forms but also its resulting colloidal stability and interaction with cell culture supplements.