Effects of Life Stage, Site, and Species on the Dragonfly Gut Microbiome
Effects of Life Stage, Site, and Species on the Dragonfly Gut Microbiome
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DOI：
10.3390/microorganisms8020183
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发表时间：
2020-02-01
期刊：
影响因子：
4.5
通讯作者：
Jackson, Colin R.
中科院分区：
文献类型：
--
作者：
Nobles, Sarah;Jackson, Colin R.
经历从幼年到成虫变态的昆虫提供了一个有趣的机会来检查生命阶段、物种和环境对其肠道微生物组的影响。在这项研究中，我们调查了从受到不同程度人类影响的 5 个不同地点收集的 13 种蜻蜓的肠道微生物组。幼虫是从水生栖息地以若虫的形式收集的，而空中成虫则是在同一地点捕获的。通过细菌 16S rRNA 基因的下一代测序来表征肠道微生物组。生命阶段是一个重要因素，蜻蜓若虫的肠道微生物组与成年蜻蜓的肠道微生物组不同。若虫的肠道微生物组受到采样地点的影响，在较小程度上也受到宿主物种的影响。样本位置和宿主物种对蜻蜓成虫的肠道微生物组都没有强烈影响。无论生命阶段如何，肠道微生物群都以变形菌门的成员为主，拟杆菌门（尤其是成虫）、厚壁菌门和酸杆菌门（尤其是若虫）的成员也相应丰富。这些结果表明，变态昆虫的不同生命阶段可以拥有非常不同的肠道微生物组，并且该微生物组受周围环境影响的方式也不同。
Insects that undergo metamorphosis from juveniles to adults provide an intriguing opportunity to examine the effects of life stage, species, and the environment on their gut microbiome. In this study, we surveyed the gut microbiomes of 13 species of dragonfly collected from five different locations subject to different levels of human impact. Juveniles were collected as nymphs from aquatic habitats while airborne adults were caught at the same locations. The gut microbiome was characterized by next generation sequencing of the bacterial 16S rRNA gene. Life stage was an important factor, with the gut microbiomes of dragonfly nymphs differing from those of adult dragonflies. Gut microbiomes of nymphs were influenced by sample site and, to a lesser extent, host species. Neither sample location nor host species had a strong effect on the gut microbiome of dragonfly adults. Regardless of life stage, gut microbiomes were dominated by members of the Proteobacteria, with members of the Bacteroidetes (especially in adults), Firmicutes, and Acidobacteria (especially in nymphs) also being proportionally abundant. These results demonstrate that different life stages of metamorphosing insects can harbor very different gut microbiomes and differ in how this microbiome is influenced by the surrounding environment.