Genomic organization of the mouse pore-forming protein (perforin) gene and localization to chromosome 10. Similarities to and differences from C9.
Genomic organization of the mouse pore-forming protein (perforin) gene and localization to chromosome 10. Similarities to and differences from C9.
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DOI：
10.1084/jem.171.2.545
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发表时间：
1990-02-01
期刊：
影响因子：
--
通讯作者：
Dupont B
中科院分区：
文献类型：
--
作者：
Trapani JA;Kwon BS;Kozak CA;Chintamaneni C;Young JD;Dupont B
包含小鼠淋巴细胞孔形成蛋白基因（Pfp）的整个编码区的基因组克隆已被分离并用于确定其内含子-外显子组织。与C9相比，Pfp具有简单的结构，仅由三个外显子（其中两个编码多肽）、一个大的5 '内含子和一个位于基因的蛋白质编码部分的大约三分之一的单个较小的内含子组成。编码PFP和C9的同源结构域的区域在C9的外显子7、8、9和10上编码，但仅形成Pfp中外显子III的开放阅读框的大约一半。虽然这两个基因编码的多肽具有相关的功能，但它们具有如此鲜明的结构，这表明它们的类似区域可能是通过趋同进化过程独立出现的。使用一组体细胞杂交细胞系，Pfp已被映射到染色体10。
Genomic clones encompassing the entire coding region of the mouse lymphocyte pore-forming protein gene (Pfp) have been isolated and used to determine its intron-exon organization. In contrast to C9, Pfp has a simple structure, consisting of only three exons (two of which encode polypeptide), a large 5' intron, and a single, smaller intron that is situated approximately one-third of the way through the protein-coding portions of the gene. The regions encoding the homologous domains of PFP and C9 are encoded on exons 7, 8, 9, and 10 of C9, but form only approximately half of the open reading frame of exon III in Pfp. Although encoding polypeptides with related functions, the two genes possess such sharply contrasting structures as to suggest that their analogous regions may have risen independently, by a process of convergent evolution. Using a panel of somatic cell hybrid cell lines, Pfp has been mapped to chromosome 10.