Mimicking Melanosomes: Polydopamine Nanoparticles as Artificial Microparasols.
Mimicking Melanosomes: Polydopamine Nanoparticles as Artificial Microparasols.
复制标题
DOI：
10.1021/acscentsci.6b00230
复制
发表时间：
2017-06-28
影响因子：
18.2
通讯作者：
Gianneschi NC
中科院分区：
文献类型：
--
作者：
Huang Y;Li Y;Hu Z;Yue X;Proetto MT;Jones Y;Gianneschi NC
黑色素在皮肤中的主要作用是防止紫外线对人体皮肤细胞造成的核DNA损伤，从而屏蔽有害的紫外线辐射。黑色素从黑色素细胞中以黑素体的形式排出后，被输送到皮肤中的角质形成细胞。人类黑色素生成缺陷可导致疾病，其中许多疾病由于其遗传来源而目前缺乏有效的治疗方法（例如皮肤癌、白癜风和白化病）。黑色素相关疾病的广泛流行和对各种与黑色素相关的聚合物材料性能的兴趣日益增加，需要新的合成路线来制备黑色素样材料。在这项工作中，我们通过多巴胺的自发氧化制备了黑色素样纳米颗粒（MelNPs），作为天然存在的黑素体的生物相容性合成类似物，并研究了它们在人角质形成细胞中的摄取、运输、分布和紫外线防护能力。关键的是，我们证明MelNPs在人表皮角质形成细胞（HEKa）中被内吞，经历核周聚集，并形成核上帽，或所谓的微遮阳伞，在细胞分布方面模仿天然黑素体的行为，并保护细胞免受紫外线损伤。在人类皮肤中，黑素小体在角质形成细胞的细胞核周围形成微型保护伞，保护DNA免受紫外线损伤。在这里，完全合成的黑色素纳米颗粒被人类角质形成细胞所接受，并被用作人工紫外线屏障。
A primary role of melanin in skin is the prevention of UV-induced nuclear DNA damage to human skin cells, where it serves to screen out harmful UV radiation. Melanin is delivered to keratinocytes in the skin after being excreted as melanosomes from melanocytes. Defects in melanin production in humans can cause diseases, many of which currently lack effective treatments due to their genetic origins (e.g., skin cancer, vitiligo, and albinism). The widespread prevalence of melanin-related diseases and an increasing interest in the performance of various polymeric materials related to melanin necessitates novel synthetic routes for preparing melanin-like materials. In this work, we prepared melanin-like nanoparticles (MelNPs) via spontaneous oxidation of dopamine, as biocompatible, synthetic analogues of naturally occurring melanosomes, and investigated their uptake, transport, distribution, and UV-protective capabilities in human keratinocytes. Critically, we demonstrate that MelNPs are endocytosed, undergo perinuclear aggregation, and form a supranuclear cap, or so-called microparasol in human epidermal keratinocytes (HEKa), mimicking the behavior of natural melananosomes in terms of cellular distribution and the fact that they serve to protect the cells from UV damage. In human skin, melanosomes form microscale umbrellas around nuclei of keratinocytes, shielding DNA from UV damage. Herein, entirely synthetic melanin nanoparticles are accepted by human keratinocytes and are used as artificial UV shields.