INDUCTION OF ERMC REQUIRES TRANSLATION OF THE LEADER PEPTIDE
INDUCTION OF ERMC REQUIRES TRANSLATION OF THE LEADER PEPTIDE
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DOI：
10.1002/j.1460-2075.1985.tb03661.x
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发表时间：
1985-01-01
期刊：
影响因子：
11.4
通讯作者：
DUBNAU, D
中科院分区：
文献类型：
--
作者：
DUBNAU, D
ErmC通过指定核糖体RNA甲基酶，导致核糖体对这些抗生素的亲和力降低，从而对大环内酯-林可酰胺链球菌素B抗生素产生耐药性。红霉素可诱导ermC的表达。提出了一个翻译调控模型，在该模型中，红霉素导致翻译先导肽的核糖体停滞。停滞导致ermC mRNA的构象改变，进而揭开甲基酶核糖体结合位点的面纱。通过用TAA替换可能的ermC前导肽编码区的第二个密码子，验证了该翻译衰减模型对ermC诱导的预测。正如预期的那样，这种突变的引入导致了一种不可诱导的表型，而这种表型可以被枯草芽孢杆菌中的两个赭石抑制基因突变所抑制。结论：ermC的诱导需要通过前导肽编码区的翻译，与预测的前导肽一致。
ermC confers resistance to macrolide-lincosamide streptogramin B antibiotics by specifying a ribosomal RNA methylase, which results in decreased ribosomal affinity for these antibiotics. ermC expression is induced by exposure to erythromycin. A translational regulation model was proposed in which erythromycin causes stalling of a ribosome, which is translating a leader peptide. Stalling causes a conformation shift in the ermC mRNA which in turn unmasks the methylase ribosomal binding site. A prediction of this translational attenuation model for ermC induction was tested by replacing the second codon of the putative ermC leader peptide coding region by TAA. As expected, the introduction of this mutation resulted in an uninducible phenotype which was suppressible by 2 ochre suppressor mutations in Bacillus subtilis. It is concluded that translation through the leader peptide coding region, in frame with the predicted leader peptide, is required for ermC induction.