Visualisation of metal deposition in biofilm reactors by three-dimensional magnetic resonance imaging (MRI)
Visualisation of metal deposition in biofilm reactors by three-dimensional magnetic resonance imaging (MRI)
复制标题
通过三维磁共振成像 (MRI) 可视化生物膜反应器中的金属沉积
作者：
K. P. Nott;M. Paterson;L. Macaskie;L. Hall
在补充有甘油 2-磷酸的柠檬酸盐缓冲液中用 La3+ 和/或 Cu2+ 激发后，使用三维磁共振成像 (MRI) 来可视化聚氨酯泡沫固定的柠檬酸杆菌。观察到广泛的磷酸酶介导的 LaPO4 生物累积，但通过 X 射线衍射和质子诱导 X 射线发射分析没有获得 Cu3(PO4)2 沉积的证据。图像分析表明La3+/Cu2+是通过MRI非侵入性研究该生物膜反应器功能的良好模型系统。
Three-dimensional magnetic resonance imaging (MRI) was used to visualise polyurethane foam-immobilised Citrobacter after challenging with La3+ and/or Cu2+ in citrate buffer supplemented with glycerol 2-phosphate. Extensive phosphatase-mediated bioaccumulation of LaPO4 was observed but no evidence for deposition of Cu3(PO4)2 was obtained by X-ray diffraction and proton-induced X-ray emission analyses. Image analysis showed that La3+/Cu2+ is a good model system to study the function of this biofilm reactor non-invasively by MRI.