The influence of low pH on in vitro growth and biochemical parameters of Plantago almogravensis and P. algarbiensis
The influence of low pH on in vitro growth and biochemical parameters of Plantago almogravensis and P. algarbiensis
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DOI：
10.1007/s11240-011-9963-1
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发表时间：
2011-05
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影响因子：
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通讯作者：
N. Martins;S. Gonçalves;T. Palma;A. Romano
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文献类型：
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作者：
N. Martins;S. Gonçalves;T. Palma;A. Romano
低培养基pH（4.50、5.00和5.75）对车前草和车前草体外生长及生化指标（脂质过氧化、脯氨酸和碳水化合物含量、抗氧化酶活性和总可溶性蛋白）的影响。对海藻的微繁殖芽进行了研究。总的来说，培养基的pH值对离体增殖和生根没有影响。有趣的是，这两个物种的培养将初始pH值修改为相同的最终值。结果表明，在最低的pH值下，两种植物的根和枝条的脂质过氧化水平均有所增加。Algarbiensis，表明质膜损伤。在根中观察到碳水化合物的积累。在pH 4.50和5.00条件下培养almogravensis。观察到酶系统对中等pH值有轻微的响应，特别是在p中。almogravensis。结果表明，车前草在体外培养条件下的pH值比组织培养条件下的pH值要低得多，这与车前草和车前草在酸性土壤中的定殖是一致的。
The effects of low medium pH (4.50, 5.00 and 5.75) on in vitro growth and on several biochemical parameters (lipid peroxidation, proline and carbohydrate content, antioxidant enzymes activities and total soluble protein) ofPlantago almogravensisandP. algarbiensismicropropagated shoots were investigated. Overall, it was observed that medium pH did not affect in vitro proliferation and rooting. Interestingly, cultures of both species modify the initial pH value to the same final value. Results have shown that the lowest pH tested induced an increase in the level of lipid peroxidation in roots of both species and in shoots ofP. algarbiensis, indicating plasma membrane damage. An accumulation of carbohydrates was observed in roots ofP. almogravensiscultured in pH 4.50 and 5.00. It was observed a slight response of the enzymatic system to medium pH, particularly inP. almogravensis. Based on the results obtained we can conclude thatPlantagospecies are apt to grow in vitro in medium with pH values much lower than the usually used in tissue culture, which is in agreement with the fact that both species colonize acid soils.