Gas-chromatographic analysis for therapeutic concentration of imipramine and disipramine in plasma, with use of a nitrogen detector.
Gas-chromatographic analysis for therapeutic concentration of imipramine and disipramine in plasma, with use of a nitrogen detector.
复制标题
使用氮气检测器对血浆中丙咪嗪和丙咪嗪的治疗浓度进行气相色谱分析。
DOI：
10.1093/clinchem/22.10.1697
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发表时间：
1976
影响因子：
9.3
通讯作者：
P. Jatlow
中科院分区：
文献类型：
--
作者：
D. Bailey;P. Jatlow
我们描述了一种用氮检测器同时测定人血浆中治疗浓度丙咪嗪和地昔帕明(活性N-脱甲基代谢物)的方法。丙咪嗪为不变碱，地西帕明为其N-三氯乙酰基衍生物。普罗马津为内标。每种药物的灵敏度下限为5µg/L，日内变异系数分别为6.0%和3.3%。我们测量了接受治疗量丙咪嗪的患者和服用过量丙咪嗪的患者的这些药物的浓度。
We describe a method for the simultaneous gas-chromatographic analysis of imipramine and desipramine (the active N-desmethyl metabolite) at therapeutic concentrations in human plasma, with use of a nitrogen detector. Imipramine is measured as the unchanged base and desipramine as its N-tribluoroacetyl derivative. Promazine is the internal standard. The lower limit of sensitivity is 5 mug/liter for each drug, and the within-day coefficients of variation for the determination of imipramine and desipramine, respectively, are 6.0% and 3.3%. We measured concentrations of these drugs both in patients receiving therapeutic doses of imipramine and in those who had ingested an overdose.