Inhibitory effects of phloroglucinols from the roots of Dryopteris crassirhizoma on melanogenesis
Inhibitory effects of phloroglucinols from the roots of Dryopteris crassirhizoma on melanogenesis
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DOI：
10.1016/j.phytol.2017.05.022
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发表时间：
2017-09-01
影响因子：
1.7
通讯作者：
Kim, Jeong Ah
中科院分区：
文献类型：
--
作者：
Van Cong Pham;Kim, Okhwa;Kim, Jeong Ah
从粗鳞毛蕨根中分离得到2个新的间苯三酚(7和10)及其12个已知的衍生物(1-6、8-9和11-14)。它们的化学结构通过各种波谱方法(H-1核磁共振、C-13核磁共振、COSY、HMQC和HMBC)和高分辨质谱学确定。检测了所有菌株(1-14)对B16F10小鼠黑色素瘤细胞黑素生成的抑制作用。去甲天冬氨酸AB(8)、去甲黄天冬氨酸PB(9)和黄酮酸PB(12)对黑素生成有抑制作用，IC50值分别为181.3、35.7和38.9mU M。化合物9对3-异丁基-1-甲基黄嘌呤(IBMX)刺激的B16F10细胞的酪氨酸酶活性和黑素生成有抑制作用，IC50值为5.9mM，呈剂量依赖关系。
Two new phloroglucinols (7 and 10), along with 12 known derivatives (1-6, 8-9, and 11-14), were isolated from the roots of Dryopteris crassirhizoma (Aspiadaceae). Their chemical structures were elucidated by various spectroscopic methods (H-1 NMR, C-13 NMR, COSY, HMQC, and HMBC) and high-resolution mass spectrometry. All isolates (1-14) were tested for their inhibitory effects on melanin production in B16F10 murine melanoma cells. Norflavaspidic acid AB (8), norflavaspidic acid PB (9), and flavaspidic acid PB (12) inhibited melanin production with IC50 values of 181.3, 35.7, and 38.9 mu M, respectively. Compound 9 was also shown to inhibit tyrosinase activity in a dose-dependent manner and melanogenesis with an IC50 value of 5.9 mu M in B16F10 cells stimulated with 3-isobutyl-1-methylxanthine (IBMX).