Distinct Mobilization of Circulating CD271+ Mesenchymal Progenitors from Hematopoietic Progenitors During Aging and After Myocardial Infarction
Distinct Mobilization of Circulating CD271+ Mesenchymal Progenitors from Hematopoietic Progenitors During Aging and After Myocardial Infarction
复制标题
DOI：
10.5966/sctm.2011-0051
复制
发表时间：
2012-06-01
影响因子：
6
通讯作者：
Suzuki, Hiroshi
中科院分区：
文献类型：
--
作者：
Iso, Yoshitaka;Yamaya, Sayaka;Suzuki, Hiroshi
间充质干细胞/祖细胞（MSCs）的特异性细胞表面标记物在体内的定义很差，但在最近的一项研究中，从人骨髓细胞的cd271阳性部分直接分离出MSC亚群。本研究的目的是鉴定人外周血中循环CD271(+) MSCs，并研究急性心肌梗死（MI）后这些细胞是否被动员。流式细胞术分析鉴定了成人外周血中的CD45（低/-）CD34(+)CD271（+）细胞。无冠状动脉疾病的老年受试者循环CD4510（低/-）CD34(+)CD133（+）细胞（造血谱系祖细胞）的数量明显低于健康的年轻受试者，而CD45（低/-）CD34(+)CD271（+）细胞的数量在老年受试者和年轻受试者之间是相当的。急性心肌梗死患者的CD45（低/-）、CD34（+）、CD271（+）和CD133（+）细胞计数均高于稳定型冠状动脉疾病患者。在我们对急性心肌梗死后时间变化的研究中，CD45（低/-）CD34(+)CD133（+）细胞计数逐渐增加到第7天。在同一时期，CD45（低/-）CD34(+)CD271（+）细胞计数在第3天达到峰值，然后下降到第7天。重要的是，急性心肌梗死后第3天的CD271（+）细胞计数与肌酸激酶峰值浓度呈正相关。本研究结果表明，CD271（+）间充质干细胞的动员方式不同于CD133（+）造血祖细胞，可能在年龄相关变化和急性心肌梗死后的组织修复过程中发挥特定作用。干细胞转化医学2012；1:462 - 468
The specific cell surface markers on mesenchymal stem/progenitor cells (MSCs) have been poorly defined in vivo, but in one recent study, an MSC subpopulation was directly isolated from a CD271-positive fraction of human bone marrow cells. The aim of this study was to identify circulating CD271(+) MSCs in human peripheral blood and investigate whether the cells are mobilized after acute myocardial infarction (MI). A flow cytometric analysis identified CD45(low/-)CD34(+)CD271(+) cells in adult human peripheral blood. The numbers of circulating CD4510(low/-)CD34(+)CD133(+) cells (hematopoietic linage progenitors) were significantly lower in elderly subjects without coronary artery disease than in healthy young subjects, whereas the numbers of CD45(low/-)CD34(+)CD271(+) cells were comparable between elderly subjects and younger subjects. The CD45(low/-)CD34(+)CD271(+) and CD133(+) cell counts were both higher in patients with acute MI than in patients with stable coronary artery disease. In our investigation of the time course changes after acute MI, the CD45(low/-)CD34(+)CD133(+) cell counts gradually increased up to day 7. Over the same period, the CD45(low/-)CD34(+)CD271(+) cell counts peaked at day 3 and then declined up to day 7. Importantly, the CD271(+) cell counts at day 3 were positively correlated with the peak concentrations of creatine kinase after acute MI. Results of the present study suggest that the CD271(+) MSCs are mobilized differently from the CD133(+) hematopoietic progenitors and may play a specific role in the tissue repair process during age-related changes and after acute myocardial infarction. STEM CELLS TRANSLATIONAL MEDICINE 2012;1:462-468