VITAMIN-D BINDING-PROTEIN SEQUESTERS MONOMERIC ACTIN IN THE CIRCULATION OF THE RAT
VITAMIN-D BINDING-PROTEIN SEQUESTERS MONOMERIC ACTIN IN THE CIRCULATION OF THE RAT
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DOI：
10.1172/jci112963
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发表时间：
1987-05-01
影响因子：
15.9
通讯作者：
HADDAD, JG
中科院分区：
文献类型：
--
作者：
HARPER, KD;MCLEOD, JF;HADDAD, JG
血浆维生素D结合蛋白（DBP）可抑制细胞裂解时释放的肌动蛋白.我们研究了125 I-DBP，125 I-G-肌动蛋白，和DBP-G-肌动蛋白复合物静脉给药后，大鼠的血浆消失和组织外观。DBP和DBP-肌动蛋白的血浆消失是不可区分的，具有快速的初始（t1/2 = 2.6 h）和较慢的第二（t1/2 = 7 h）斜率。注射125 I-G-actin（纳摩尔）后，血浆消失率超过DBP和DBP-actin。所有注射的肌动蛋白与DBP相关，没有证据表明游离肌动蛋白，肌动蛋白凝溶胶蛋白复合物或肌动蛋白寡聚体。125 I-载脂蛋白（apo）或holo-DBP的组织外观相似，在灌注的肝脏，肾脏和骨骼肌中积累最多。尽管细胞溶解后体内会发生更复杂的现象（F-肌动蛋白和细胞内肌动蛋白结合蛋白进入血浆），但我们的结果表明DBP在循环中肌动蛋白单体的封存和处置中发挥作用。
Plasma vitamin D binding protein (DBP) may scavenge actin released during cell lysis. We examined the plasma disappearance and tissue appearance of 125I-DBP, 125I-G-actin, and the DBP-G-actin complex after their intravenous administration to rats. The plasma disappearance of DBP and DBP-actin were indistinguishable, with rapid initial (t1/2 = 2.6 h) and slower second (t1/2 = 7 h) slopes. After 125I-G-actin (nanomole) injection, plasma disappearance paralleled that of DBP and DBP-actin. All injected actin was associated with DBP, without evidence of free actin, actin-gelsolin complexes or actin oligomers. Tissue appearances of 125I-apolipoprotein (apo) or holo-DBP were similar, with highest accumulations in perfused liver, kidney, and skeletal muscle. Although more complex phenomena (plasma entry of F-actin and intracellular actin binding proteins) would occur in vivo after cell lysis, our results suggest a role for DBP in the sequestration and disposition of actin monomers in the circulation.