Human peripheral blood dendritic cells and monocyte subsets display similar chemokine receptor expression profiles with differential migratory responses
Human peripheral blood dendritic cells and monocyte subsets display similar chemokine receptor expression profiles with differential migratory responses
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DOI：
10.1111/j.1365-3083.2007.01933.x
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发表时间：
2007-06-01
影响因子：
3.7
通讯作者：
Lipsky, P. E.
中科院分区：
文献类型：
--
作者：
Cravens, P. D.;Hayashida, K.;Lipsky, P. E.
在外周血中发现的人抗原呈递细胞（APC）被认为是已经从骨髓释放并转运到外周组织的前体。这些APC群体包括髓样树突状细胞（mDC）、浆细胞样DC（pDC）和单核细胞（Mo）。为了指定APC的专门功能作用和发育阶段，检测了表达CD 33的APC亚群对趋化因子的应答能力。CD 33（+）主要有3个亚群：CD 33（bright）CD 14（bright）Mo、CD 33（bright）CD 14（-）CD 11 c（+）mDC和CD 33（dim）CD 14（-）pDC。树突状细胞亚群和钼表达低水平的CC和CXC受体，但没有观察到独特的趋化因子受体表达谱。表达特定趋化因子受体的细胞百分比在不同供体之间以及在同一供体中随时间变化。髓样DC和Mo但不是pDC以浓度依赖性方式向CXCL 12迁移。单核细胞和pDC，但不是髓样DC，被高浓度的CXCL 10吸引。所有CD 33（+）亚群均以浓度依赖性方式向CCL 19迁移，但对CCL 21的反应较弱。CCL 20对任何种群都不是化学引诱物。尽管发现APC没有表现出独特的表面趋化因子受体表达模式，但它们表现出向CXCL 12、CXCL 10和CCL 21的差异性迁移，而不是向CCL 20或CCL 19的差异性迁移。
Human antigen presenting cells (APC) found in peripheral blood are considered to be precursors that have been released from the bone marrow and are in transit to the peripheral tissues. These APC populations include myeloid dendritic cells (mDC), plasmacytoid DC (pDC) and monocytes (Mo). To assign specialized functional roles and stages of development for APCs, CD33 expressing APC subsets were examined for their capacity to respond to chemokines. Three major CD33(+) subsets including CD33(bright)CD14(bright) Mo, CD33(bright)CD14(-) CD11c(+) mDC and CD33(dim)CD14(-) pDC were present. Dendritic cells subsets and Mo expressed low levels of CC and CXC receptors, but distinctive chemokine receptor expression profiles were not observed. The percentage of cells expressing a particular chemokine receptor varied from donor to donor and over time in the same donor. Myeloid DC and Mo but not pDC migrated toward CXCL12 in a concentration dependent manner. Monocytes and pDC, but not myeloid DC, were attracted by high concentrations of CXCL10. All CD33(+) subsets migrated in a concentration dependent manner toward CCL19, but responded less robustly to CCL21. CCL20 was not chemoattractant for any population. Despite the finding that APC did not exhibit unique surface chemokine receptor expression patterns, they exhibited differential migration to CXCL12, CXCL10 and CCL21 but not to CCL20 or CCL19.