Different expression patterns of Gli1-3 in mouse embryonic maxillofacial development
Different expression patterns of Gli1-3 in mouse embryonic maxillofacial development
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Gli1-3在小鼠胚胎颌面部发育中的不同表达模式
DOI：
10.1016/j.acthis.2011.11.007
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发表时间：
2012-01-01
影响因子：
2.5
通讯作者：
Wang, Songlin
中科院分区：
文献类型：
--
作者：
Du, Juan;Fan, Zhipeng;Wang, Songlin
Sonic hedgehog（SHH）信号通路在果蝇和脊椎动物胚胎发育中起着许多关键作用，包括调节颅面发育。转录因子GLI家族（GLI 1、2和3）通过调节下游靶基因的表达介导SHH形态发生信号。信号异常严重影响脊椎动物的发育。为了更好地了解GLI转录因子的调控，我们研究了Gli 1 -3在小鼠胚胎颅面发育过程中的表达模式，采用原位杂交与整体和切片和定量实时PCR。我们发现，Gli 1的表达主要是在胚胎真皮间充质中检测到的，并强烈检测到在胚胎口腔粘膜在面部发育的后期阶段。Gli 2在真皮和口腔粘膜的间充质中表达较强。Gli 3的表达检测微弱的上皮细胞比间充质在11天后cobaly（dpc），但在14.5 dpc，在上皮细胞中的表达更强。在这三个基因中，Gill表达在面部发育的早期阶段最高，Gli 3表达在面部发育的后半段最高（此后保持稳定），而Gli 2表达在整个面部发育过程中保持低水平。本研究结果表明，Gli 1 -3可能在面部发育过程中发挥不同的作用。(C)2011年Elsevier GmbH。All rights reserved.
The Sonic hedgehog (SHH) signaling pathway plays many key roles in the development of Drosophila and vertebrate embryos, including regulating craniofacial development. The GLI family of transcription factors (GLI1, 2, and 3) mediates the SHH morphogenetic signal by regulating the expression of downstream target genes. Signaling aberrations seriously affect vertebrate development. To better understand the regulation of GLI transcription factors, we investigated the expression patterns of Gli1-3 during murine embryonic craniofacial development using in situ hybridization with whole-mounts and sections and quantitative real-time PCR. We found that Gli1 expression was mostly detected in the mesenchyme in embryonic dermis, and strongly detected in embryonic oral mucosa at late stages of face development. Gli2 was strong in mesenchyme, both in dermis and oral mucosa. Gli3 expression was detected fainter in the epithelium than the mesenchyme at 11 days post-coitum (dpc), but at 14.5 dpc, expression was stronger in the epithelium. Of the three genes, Gill expression was highest in early stages of face development, Gli3 expression was highest in the second half of facial development (and remained stable thereafter), whereas Gli2 expression remained low throughout facial development. The present findings suggest that Gli1-3 may play different roles during facial development. (C) 2011 Elsevier GmbH. All rights reserved.