Fractal dimension of microbead assemblies used for protein detection.
Fractal dimension of microbead assemblies used for protein detection.
复制标题
用于蛋白质检测的微珠组件的分形维数。
DOI：
10.1002/cphc.201402048
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Kopelman,Raoul
中科院分区：
文献类型：
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作者：
Hecht,Ariel;Commiskey,Patrick;Lazaridis,Filippos;Argyrakis,Panos;Kopelman,Raoul
我们利用分形分析方法计算了1 μm微球旋转磁性组装体中的蛋白质浓度，优化了沉降、结合位点和磁性体积参数。我们使用原始的Forrest-Witten方法，但由于头部粒子的数量相对较少，只有500个数量级，因此我们使用了大量的原点和大量的算法迭代。我们发现分形维数的值在1.70-1.90范围内，作为凝血酶浓度的函数，凝血酶起着将微珠结合在一起的作用。这与以前的磁旋研究结果很好地吻合。多参考点分形维数的计算可用于任何粒子数量相对较少的装配。
We use fractal analysis to calculate the protein concentration in a rotating magnetic assembly of microbeads of size 1 μm, which has optimized parameters of sedimentation, binding sites and magnetic volume. We utilize the original Forrest–Witten method, but due to the relatively small number of bead particles, which is of the order of 500, we use a large number of origins and also a large number of algorithm iterations. We find a value of the fractal dimension in the range 1.70–1.90, as a function of the thrombin concentration, which plays the role of binding the microbeads together. This is in good agreement with previous results from magnetorotation studies. The calculation of the fractal dimension using multiple points of reference can be used for any assembly with a relatively small number of particles.