Genetic characterization of a betanodavirus isolated from a clinical disease outbreak in farm-raised tilapia Oreochromis niloticus (L.) in Thailand
Genetic characterization of a betanodavirus isolated from a clinical disease outbreak in farm-raised tilapia Oreochromis niloticus (L.) in Thailand
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DOI：
10.1111/jfd.12200
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发表时间：
2015-01-01
影响因子：
2.5
通讯作者：
Pirarat, N.
中科院分区：
文献类型：
--
作者：
Keawcharoen, J.;Techangamsuwan, S.;Pirarat, N.
在泰国中部养殖的罗非鱼幼体中发现了Betanoda病毒感染。免疫组织化学染色阳性的组织切片上可见广泛的空泡变性和神经元坏死。根据衣壳蛋白基因的核苷酸序列(1333个碱基)进行了分子系统发育分析。该毒株与赤点石斑鱼神经坏死病毒(RGNNV)有很高的同源性(93.07-93.88%)，且与RGNNV关系密切。
Betanodavirus infection was diagnosed in larvae of farm-raised tilapia Oreochromisniloticus (L.), in central Thailand. Extensive vacuolar degeneration and neuronal necrosis were observed in histological sections with positive immunohistochemical staining for betanodavirus. Molecular phylogenetic analysis was performed based on the nucleotide sequences (1333 bases) of the capsid protein gene. The virus strain was highly homologous (93.07-93.88%) and closely related to red-spotted grouper nervous necrosis virus (RGNNV).