GENERATION OF A HYBRID SEQUENCE-SPECIFIC SINGLE-STRANDED DEOXYRIBONUCLEASE
GENERATION OF A HYBRID SEQUENCE-SPECIFIC SINGLE-STRANDED DEOXYRIBONUCLEASE
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DOI：
10.1126/science.3685986
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发表时间：
1987-12-04
期刊：
影响因子：
56.9
通讯作者：
SCHULTZ, PG
中科院分区：
文献类型：
--
作者：
COREY, DR;SCHULTZ, PG
相对非特异性的单链脱氧核糖核酸酶，葡萄球菌核酸酶，选择性地融合到一个寡核苷酸结合位点的定义序列，以产生一个杂交酶。半胱氨酸取代的Lys 116在酶中通过阿托伐他汀定向诱变和偶联的寡核苷酸，含有3“-硫醇。所得的杂合酶在与寡核苷酸结合位点相邻的位点处切割单链DNA。
The relatively nonspecific single-stranded deoxyribonuclease, staphylococcal nuclease, was selectively fused to an oligonucleotide binding site of defined sequence to generate a hybrid enzyme. A cysteine was substituted for Lys116 in the enzyme by oligonucleotide-directed mutagenesis and coupled to an oligonucleotide that contained a 3''-thiol. The resulting hybrid enzyme cleaved single-stranded DNA at sites adjacent to the oligonucleotide binding site.