A topographic map of recruitment in spinal cord
A topographic map of recruitment in spinal cord
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DOI：
10.1038/nature05588
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发表时间：
2007-03-01
期刊：
影响因子：
64.8
通讯作者：
Fetcho, Joseph R.
中科院分区：
文献类型：
--
作者：
McLean, David L.;Fan, Jingyi;Fetcho, Joseph R.
动物通过使用位于脊髓的有节奏的神经元网络在一定的速度范围内移动(1-6)。在这里，我们使用电生理学和活体成像在斑马鱼幼体(Danio Rerio)身上揭示了脊髓神经元的位置与神经元活动的最小游泳频率之间的系统关系。腹侧运动神经元和兴奋性中间神经元在最低的游泳频率下有节奏地活动，随着游泳频率的增加，越来越多的背部兴奋性神经元参与其中。抑制性中间神经元遵循相反的模式。这些倒置的募集模式与中间神经元之间的胞体大小无关，但可能部分地由伴随的输入阻力的背腹梯度来解释。激光消融腹侧而不是背侧的兴奋性中间神经元扰乱缓慢的运动，支持地形图的行为作用。我们的结果揭示了斑马鱼脊髓内一种意想不到的组织模式，这是产生不同速度运动的基础。
Animals move over a range of speeds by using rhythmic networks of neurons located in the spinal cord(1-6). Here we use electrophysiology and in vivo imaging in larval zebrafish ( Danio rerio) to reveal a systematic relationship between the location of a spinal neuron and the minimal swimming frequency at which the neuron is active. Ventral motor neurons and excitatory interneurons are rhythmically active at the lowest swimming frequencies, with increasingly more dorsal excitatory neurons engaged as swimming frequency rises. Inhibitory interneurons follow the opposite pattern. These inverted patterns of recruitment are independent of cell soma size among interneurons, but may be partly explained by concomitant dorso-ventral gradients in input resistance. Laser ablations of ventral, but not dorsal, excitatory interneurons perturb slow movements, supporting a behavioural role for the topography. Our results reveal an unexpected pattern of organization within zebrafish spinal cord that underlies the production of movements of varying speeds.