Estimating the per-base-pair mutation rate in the yeast Saccharomyces cerevisiae
Estimating the per-base-pair mutation rate in the yeast Saccharomyces cerevisiae
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DOI：
10.1534/genetics.107.071506
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发表时间：
2008-01-01
期刊：
影响因子：
3.3
通讯作者：
Murray, Andrew W.
中科院分区：
文献类型：
--
作者：
Lang, Gregory I.;Murray, Andrew W.
尽管突变率是进化速率的关键决定因素，但它们很难精确测量，并且全局突变率（每代每个基因组的突变）通常是从每个碱基对的突变率推断出来的，假设突变率在整个基因组中是一致的。使用芽殖酵母，我们描述了基于波动测定精确计算突变率的所有改进方法。我们的分析表明。两个基因的每碱基对突变率显着不同（URA3 为 3.80 X 10(-10)），CAN1 为 6.44 X 10(-10)），我们提出了基因有效目标大小的定义（突变使基因失活的概率），承认整个基因组的起始率是不均匀的。
Although mutation rates are a key determinant of the rate of evolution they are difficult to measure precisely and global mutations rates mutations per genome per generation) are often extrapolated from the per-base-pair Mutation rate assuming that mutation rate is uniform across the genome. Using budding yeast, we describe all improved method for the accurate calculation of mutation rates based on the fluctuation assay. Our analysis suggests that. the per-base-pair mutation rates at two genes differ significantly (3.80 X 10(-10)) at URA3 and 6.44 X 10(-10)) at CAN1) and we propose a definition for the effective target size of genes (the probability that it mutation inactivates the gene) that acknowledges that the initiation rate is nonuniform across the genome.