Involvement of ganglioside GT1b in glutamate release from neuroblastoma cells.
Involvement of ganglioside GT1b in glutamate release from neuroblastoma cells.
复制标题
神经节苷脂 GT1b 参与神经母细胞瘤细胞谷氨酸的释放。
DOI：
10.1016/j.neulet.2012.04.049
复制
发表时间：
2012
影响因子：
2.5
通讯作者：
Iwabuchi K
中科院分区：
文献类型：
--
作者：
Watanabe S;Higashi H;Ogawa H;Takamori K;Iwabuchi K
由于神经节苷脂在神经系统中起着重要的作用，我们研究了神经节苷脂是否参与了神经细胞中谷氨酸的释放。用神经节苷脂GM3、GM1、GD1a、GD3、GD1b或GT1b处理分化的神经2a细胞30min，用邻苯二甲醛衍生法测定培养液中谷氨酸浓度。在被测试的神经节苷脂中，GT1b与未处理的细胞相比显著增加了谷氨酸浓度。此外，GT1b还能增加神经母细胞瘤×背根神经节神经元杂交F11细胞培养液中的谷氨酸浓度。这些结果提示神经节苷脂在调节神经系统细胞外谷氨酸浓度中起重要作用。
Since gangliosides play many important roles in neural systems, we investigated whether gangliosides are involved in glutamate release from neural cells. Differentiated neruro2a cells were treated with gangliosides, including GM3, GM1, GD1a, GD3, GD1b, or GT1b, for 30min, and glutamate concentration in the culture media was measured using o-phthalaldehyde derivatization. Among the tested gangliosides, GT1b significantly increased the glutamate concentration when compared with untreated cells. Moreover, GT1b increased the glutamate concentration in the culture media of neuroblastoma×dorsal root ganglion neuron hybrid F11 cells. These results suggested that gangliosides are important in regulating extracellular glutamate concentration in the nervous system.