LASTING POTENTIATION OF INHIBITION IS ASSOCIATED WITH AN INCREASED NUMBER OF GAMMA-AMINOBUTYRIC-ACID TYPE-A RECEPTORS ACTIVATED DURING MINIATURE INHIBITORY POSTSYNAPTIC CURRENTS
LASTING POTENTIATION OF INHIBITION IS ASSOCIATED WITH AN INCREASED NUMBER OF GAMMA-AMINOBUTYRIC-ACID TYPE-A RECEPTORS ACTIVATED DURING MINIATURE INHIBITORY POSTSYNAPTIC CURRENTS
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DOI：
10.1073/pnas.91.16.7698
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发表时间：
1994-08-02
影响因子：
11.1
通讯作者：
MODY, I
中科院分区：
文献类型：
--
作者：
OTIS, TS;DE KONINCK, Y;MODY, I
全细胞膜片钳记录揭示了一个大幅度增加的幅度，但没有变化的频率，在齿状回颗粒细胞的微型抑制性突触后电流（mIPSCs）慢性点燃诱导癫痫。这种新的和持久的增强γ-氨基丁酸A型（GABA（A））受体介导的抑制持续至少48小时后，其诱导。使用非平稳波动分析可以证明在mIPSC期间激活的功能性突触后GABA（A）受体通道的数量几乎加倍，而单通道电导或动力学没有任何变化。由于突触后GABA（A）受体可能会被间隙中的递质浓度所饱和，因此增加功能性受体通道的数量可能是增强哺乳动物脑中抑制的最有效手段。
Whole-cell patch-clamp recordings unveiled a substantial increase in the amplitude, but no change in the frequency, of miniature inhibitory postsynaptic currents (mIPSCs) in dentate gyrus granule cells following chronic epilepsy induced by kindling. This novel and persistent enhancement of gamma-aminobutyric acid type A (GABA(A)) receptor-mediated inhibition lasted for at least 48 hr following its induction. Nearly a doubling of the number of activated functional postsynaptic GABA(A) receptor channels during mIPSCs without any change in single-channel conductance or kinetics could be demonstrated using nonstationary fluctuation analysis. As postsynaptic GABA(A) receptors are likely to be pharmacologically saturated by the transmitter concentration in the cleft, incrementing the number of functional receptor channels may be the most effective means to augment inhibition in the mammalian brain.