THE EFFECT OF THE HIGH LYSINE GENES OF THE BARLEY MUTANTS RISO 1508 AND 527 ON EMBRYO DEVELOPMENT
THE EFFECT OF THE HIGH LYSINE GENES OF THE BARLEY MUTANTS RISO 1508 AND 527 ON EMBRYO DEVELOPMENT
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DOI：
10.1111/j.1399-3054.1986.tb03374.x
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发表时间：
1986-11-01
影响因子：
6.4
通讯作者：
OLSEN, OA
中科院分区：
文献类型：
--
作者：
DEGGERDAL, A;KLEMSDAL, SS;OLSEN, OA
大麦（Hordeum vulgare L.）CV. Bomi和高赖氨酸突变体527、1508和双突变体27/1508的平均产量分别为1.00、1.13、1.56和2.22 mg。通过光镜、电镜和双向凝胶电泳对四个品系的胚进行了比较。形态差异仅限于小盾组织，在1508和双突变体中具有不规则的形式。34天胚的平均小盾板细胞体积。Bomi，突变体527、1508和527/1508分别为4，200、8，300、4，900和23，400 μ m3。突变体527的小盾叶薄壁细胞淀粉含量略高于cv。Bomi，而在突变体1508和双突变体中相当高。双向凝胶电泳结果表明，突变体胚中有7个蛋白质点的大小与普通栽培种胚中的蛋白质点有显著差异。波米在这7种蛋白质中，有1种蛋白质仅在双突变体中表达，有4种蛋白质在突变体中的表达量比在普通小麦中的表达量高。波密和二号则不那么丰富。这些蛋白质的等电点和分子量并不完全对应于先前描述的大麦种子中的蛋白质。
Dry weight of 34-day-old embryos from barley (Hordeum vulgare L.) cv. Bomi and high lysine mutants 527, 1508 and the double mutant 27/1508 were 1.00, 1.13, 1.56 and 2.22 mg, respectively. Embryos of the four lines were compared by light and electron microscopy and two dimensional gel electrophoresis. Morphological differences were restricted to the scutellar tissue, which had an irregular form in 1508 and the double mutant. Average scutellar cell volumes in 34-day-old embryos from cv. Bomi, mutants 527, 1508, and 527/1508 were 4,200, 8,300, 4,900 and 23,400 .mu.m3, respectively. The starch content of the scutellar parenchymal cells was slightly higher in mutant 527 than in cv. Bomi, and considerably higher in mutant 1508 and the double mutant. The two dimensional gel electrophoretic examination showed that seven of the embryo protein spots were of a significantly different size in the mutants relative to those from cv. Bomi. Among these seven proteins, one was apparent only in the double mutant, four were more abundant in the mutants than in cv. Bomi and two were less abundant. The isoelectric points and the molecular weights of these proteins do not correspond exactly to those of proteins previously described in barley seeds.