An in vitro coupled transcription-translation system from Staphylococcus aureus.
An in vitro coupled transcription-translation system from Staphylococcus aureus.
复制标题
来自金黄色葡萄球菌的体外偶联转录翻译系统。
DOI：
10.1016/0378-1119(91)90562-p
复制
发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
Khan,SA
中科院分区：
文献类型：
--
作者：
Mahmood,R;Compagnone-Post,P;Khan,SA
We have developed aStaphylococcus aureuscell-free system that is capable of directing DNA-dependent synthesis of proteins. The staphylococcal plasmids pE194 and pSK.265 were used to characterize this system. The in vitro system was found to direct the synthesis of the appropriate proteins predicted from the nucleotide sequence of the plasmids. As is the case in vivo, low levels of the inducer, erythromycin, promoted the synthesis of the pE194-encoded ribosomal RNA methyltransferase in the in vitro system.
