Validation study to compare effects of processing protocols on measured Nε-(carboxymethyl)lysine and Nε-(carboxyethyl)lysine in blood
Validation study to compare effects of processing protocols on measured Nε-(carboxymethyl)lysine and Nε-(carboxyethyl)lysine in blood
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DOI：
10.3164/jcbn.13-5
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发表时间：
2013-11-01
影响因子：
2.4
通讯作者：
Cuskelly, Geraldine J.
中科院分区：
文献类型：
--
作者：
Hull, George L. J.;Woodside, Jayne V.;Cuskelly, Geraldine J.
流行病学研究表明，晚期糖基化终产物（AGEs）血浆水平升高与糖尿病、肾脏疾病和心脏病相关。因此，AGEs已被用作疾病进展标志物。然而，尚未研究生物样品处理程序的变化对血浆/血清样品中AGEs水平的影响。本研究的目的是评估血液样本采集的变化对测量的N-α-（羧甲基）赖氨酸（CML）（最佳特征的AGE）及其同系物N-α-（羧乙基）赖氨酸（CEL）的影响。该研究检查了不同采血管对CML和CEL的影响，包括稳定混合物，冻融循环的影响，不同的储存时间和温度，以及延迟离心对健康志愿者合并样本的影响。CML和CEL提取样品中测定超高效液相色谱-串联质谱法。中位CML和CEL的范围分别为0.132至0.140 mM/M lys和0.053至0.060 mM/M lys。CML和CEL在血浆/血清样本中无显著差异。因此，作为流行病学研究的一部分采集的样本（采集时未进行特定样本处理）适用于测量CML和CEL。
Epidemiological studies show that elevated plasma levels of advanced glycation end products (AGEs) are associated with diabetes, kidney disease, and heart disease. Thus AGEs have been used as disease progression markers. However, the effects of variations in biological sample processing procedures on the level of AGEs in plasma/serum samples have not been investigated. The objective of this investigation was to assess the effect of variations in blood sample collection on measured N-epsilon-(carboxymethyl)lysine (CML), the best characterised AGE, and its homolog, N-epsilon-(carboxyethyl)lysine (CEL). The investigation examined the effect on CML and CEL of different blood collection tubes, inclusion of a stabilising cocktail, effect of freeze thaw cycles, different storage times and temperatures, and effects of delaying centrifugation on a pooled sample from healthy volunteers. CML and CEL were measured in extracted samples by ultra-performance liquid chromatography-tandem mass spectrometry. Median CML and CEL ranged from 0.132 to 0.140 mM/M lys and from 0.053 to 0.060 mM/M lys, respectively. No significant difference was shown CML or CEL in plasma/serum samples. Therefore samples collected as part of epidemiological studies that do not undergo specific sample treatment at collection are suitable for measuring CML and CEL.