Isolation of a rice endophytic bacterium, Pantoea sp Sd-1, with ligninolytic activity and characterization of its rice straw degradation ability
Isolation of a rice endophytic bacterium, Pantoea sp Sd-1, with ligninolytic activity and characterization of its rice straw degradation ability
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具有木质素分解活性的水稻内生细菌 Pantoea sp Sd-1 的分离及其稻秆降解能力的表征
DOI：
10.1111/lam.12163
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发表时间：
2014
影响因子：
2.4
通讯作者：
Zhu Y. H.
中科院分区：
文献类型：
--
作者：
Xiong X. Q.;Liao H. D.;Ma J. S.;Liu X. M.;Zhang L. Y.;Shi X. W.;Yang X. L.;Lu X. N.;Zhu Y. H.
这项研究的重点是内生细菌菌株PantoEASP。 SD -1，可用于降解木质素和稻草。通过优化的表面灭菌方法从水稻种子中分离出这种菌株。 SD -1具有降解稻草和木质素的特殊能力。在含1％葡萄糖和0·5％肽的含稻草或牛皮纸木质素培养基中，Pantoeasp。治疗6天后，SD -1有效地将稻草质量重量降低了54·54％。孵育4天后，该菌株还能够降低木质素颜色（52·4％）和含量（69·1％）。研究结果表明，水稻内生细菌。 SD -1可以用于降解木质纤维素生物量的降解，例如稻草。在中国丰富的农业副产品研究稻草的意义和影响，由于其高木质素含量很难降解。由于细菌的巨大环境适应性和生化多功能性，内生细菌是生物降解的有用资源。在这项研究中，我们筛选了能够生物降解稻草和木质素的内生细菌，并获得了一种菌株。 SD -1，具有合适的特征。 SD -1可用于稻草和木质素的降解，并且可能在这种农业副产品的生物降解中起重要作用。
This study focused on an endophytic bacterial strain,Pantoeasp. Sd‐1, which can be used to degrade lignin and rice straw. This strain was isolated from rice seeds by an optimized surface sterilization method.Pantoeasp. Sd‐1 showed exceptional ability to degrade rice straw and lignin. In rice straw or kraft lignin‐containing medium supplemented with 1% glucose and 0·5% peptone,Pantoeasp. Sd‐1 effectively reduced the rice straw mass weight by 54·5% after 6 days of treatment. The strain was also capable of reducing the lignin colour (52·4%) and content (69·1%) after 4 days of incubation. The findings suggested that the rice endophytic bacteriumPantoeasp. Sd‐1 could be applied for the degradation of lignocellulose biomass, such as rice straw.Significance and Impact of the StudyRice straw, an abundant agricultural by‐product in China, is very difficult to degrade because of its high lignin content. Due to the immense environmental adaptability and biochemical versatility of bacteria, endophytic bacteria are useful resources for biodegradation. In this study, we screened for endophytic bacteria capable of biodegrading rice straw and lignin and obtained one strain,Pantoeasp. Sd‐1, with suitable characteristics. Sd‐1 could be used for degradation of rice straw and lignin, and may play an important role in biodegradation of this agricultural by‐product.