Amplified and overexpressed epidermal growth factor receptor gene in uncultured primary human breast carcinoma.
Amplified and overexpressed epidermal growth factor receptor gene in uncultured primary human breast carcinoma.
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发表时间：
1988
期刊：
影响因子：
11.2
通讯作者：
Jungsil Ro;Susan M. North;G. Gallick;G. Hortobagyi;Jordan U. Gutterman;M. Blick
中科院分区：
文献类型：
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作者：
Jungsil Ro;Susan M. North;G. Gallick;G. Hortobagyi;Jordan U. Gutterman;M. Blick
我们分析了表皮生长因子受体基因的互补DNA探针的表皮生长因子受体基因在21个未培养的原发性乳腺癌，发现该基因在这些肿瘤中的三个扩增。我们进一步证明了免疫组织化学使用单克隆抗体的表皮生长因子受体，该基因的受体蛋白产物过表达，并显示升高的激酶活性。我们的数据表明，表皮生长因子受体在人类乳腺癌中过度表达的分子机制之一是表皮生长因子受体基因扩增，而没有重排的肿瘤的子集。
We analyzed the epidermal growth factor receptor gene using a complementary DNA probe of the epidermal growth factor receptor gene in 21 uncultured primary breast carcinomas and found that the gene was amplified in three of these tumors. We further demonstrated by immunohistochemistry using a monoclonal antibody to the epidermal growth factor receptor that the receptor protein product of this gene was overexpressed and displayed elevated kinase activity. Our data indicate that one of the molecular mechanisms for overexpression of epidermal growth factor receptor in human breast cancer is epidermal growth factor receptor gene amplification without rearrangement in a subset of tumors.