Differential effects of carotenoids on lipid peroxidation due to membrane interactions: X-ray diffraction analysis
Differential effects of carotenoids on lipid peroxidation due to membrane interactions: X-ray diffraction analysis
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DOI：
10.1016/j.bbamem.2006.09.010
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发表时间：
2007-01-01
影响因子：
3.4
通讯作者：
Mason, R. Preston
中科院分区：
文献类型：
--
作者：
McNulty, Hyesun P.;Byun, Jungsoo;Mason, R. Preston
某些类胡萝卜素的生物学益处可能是由于它们与膜的特定物理化学相互作用所导致的有效的抗氧化特性。为了验证这一假设，我们测量了各种类胡萝卜素对脂质过氧化速率的影响，并将这些发现与它们的膜相互作用（通过小角X射线衍射方法确定）相关联。在富含多不饱和脂肪酸的膜中，评价了纯手性类胡萝卜素（虾青素、玉米黄质、叶黄素、β-胡萝卜素、番茄红素）对脂质过氧化氢（LOOH）生成的影响。非极性类胡萝卜素，如番茄红素和β-胡萝卜素，使膜双层紊乱并显示出有效的促氧化作用（LOOH水平增加> 85%），而虾青素保持膜结构并显示出显著的抗氧化活性（LOOH水平降低40%）。这些结果表明，类胡萝卜素对脂质过氧化作用的不同影响，由于膜结构的变化。类胡萝卜素对脂质过氧化的这些对比作用可能解释了它们生物活性的差异。(c)2006 Elsevier B. V.保留所有权利。
The biological benefits of certain carotenoids may be due to their potent antioxidant properties attributed to specific physico-chemical interactions with membranes. To test this hypothesis, we measured the effects of various carotenoids on rates of lipid peroxidation and correlated these findings with their membrane interactions, as determined by small angle X-ray diffraction approaches. The effects of the homochiral carotenoids (astaxanthin, zeaxanthin, lutein, beta-carotene, lycopene) on lipid hydroperoxide (LOOH) generation were evaluated in membranes enriched with polyunsaturated fatty acids. Apolar carotenoids, Such as lycopene and beta-carotene, disordered the membrane bilayer and showed a potent pro-oxidant effect (> 85% increase in LOOH levels) while astaxanthin preserved membrane structure and exhibited significant antioxidant activity (40% decrease in LOOH levels). These findings indicate distinct effects of carotenoids on lipid peroxidation due to membrane structure changes. These contrasting effects of carotenoids on lipid peroxidation may explain differences in their biological activity. (c) 2006 Elsevier B.V. All rights reserved.