Establishment and characterization of a new triple-negative canine mammary cancer cell line
Establishment and characterization of a new triple-negative canine mammary cancer cell line
复制标题
新的三阴性犬乳腺癌细胞系的建立和表征
DOI：
10.1016/j.tice.2018.07.003
复制
发表时间：
2018-10-01
期刊：
影响因子：
2.6
通讯作者：
Lin, Degui
中科院分区：
文献类型：
--
作者：
Zhang, Hong;Pei, Shimin;Lin, Degui
犬乳腺肿瘤（CMT）一直是人类乳腺癌（HBC）研究的理想动物模型，然而，缺乏与人类乳腺癌细胞系相对应的各种已建立的犬乳腺肿瘤细胞系。本研究旨在建立一种新型的犬乳腺肿瘤细胞系。原发性肿瘤CMT - 7364被鉴定为导管内乳头状癌，通过免疫组织化学（IHC）分析显示其对雌激素受体（ER）、孕激素受体（PR）和人表皮生长因子受体 - 2（HER - 2）呈免疫阴性反应。源自该原发性肿瘤的CMT - 7364细胞系对ER、PR和HER - 2也呈免疫阴性反应，并且通过免疫细胞化学（ICC）分析显示其对上皮细胞标志物呈阴性，对间充质细胞标志物呈阳性。该细胞系已稳定培养超过115代，通过ICC分析显示其具有上皮来源特征且表达上皮抗原，通过Transwell分析显示其具有侵袭能力。在体内，分别将CMT - 7364细胞接种到裸鼠模型和免疫健全小鼠模型后，发现了肺部的肿瘤肿块和转移灶。通过IHC分析，异种移植肿瘤组织对ER、PR和HER - 2也呈阴性。因此，成功建立了一种新型的三阴性犬乳腺癌细胞系CMT - 7364，它可作为犬和人类三阴性乳腺癌免疫治疗及上皮 - 间质转化（EMT）机制研究的有前景的模型。
Canine mammary tumor (CMT) has always been an ideal animal model for human breast cancer (HBC) research, however, there is a lack of various established CMT cell lines corresponding to HBC cell lines. This study was designed to establish a new type of CMT cell line. The primary tumor, CMT-7364, was identified as the intraductal papillary carcinoma, and showed negative immunoreactivity to estrogen receptors (ER), progesterone receptors (PR), and human epidermal growth factor receptor-2 (HER-2) by immunohistochemistry (IHC) analysis. The CMT-7364 cell line from this primary tumor also shows a negative immunoreactivity to ER, PR, and HER-2, and was negative to epithelial cell markers and positive to mesenchymal cell markers by immunocytochemistry (ICC) analysis. This cell line, which has been stably cultured for more than 115 passages, and was characterized by epithelial origin with the expression of the epithelial antigen by ICC analysis and invasion ability by transwell analysis. In vivo, tumor mass and metastases in the lung were found after inoculating the CMT-7364 cells in the nude mice model, and the immune-complete mice model respectively. The tissues from the xenograft tumor were also negative to ER, PR, and HER-2 by IHC analysis. Thus, a novel triple negative canine mammary cancer cell line, CMT-7364, was successfully established, which could be used as a promising model for the research of immunotherapy and Epithelial-Mesenchymal Transition (EMT) mechanism of the triple-negative breast cancer both in canine and human.