Rapid genetic mapping in Neurospora crassa
Rapid genetic mapping in Neurospora crassa
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DOI：
10.1016/j.fgb.2006.09.002
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发表时间：
2007-06-01
影响因子：
3
通讯作者：
Versaw, Wayne K.
中科院分区：
文献类型：
--
作者：
Jin, Yuan;Allan, Sabrina;Versaw, Wayne K.
正向遗传分析是目前应用最广泛的基因功能识别方法。然而，对于一些生物，如丝状子囊菌粗糙脉孢菌，遗传图谱往往是向前遗传方法的一个限制性步骤。我们描述了一个有效的方法，遗传定位在N。crassa，利用改良的散装分离分析和基于PCR的分子标记。该方法能够利用来自单次杂交的后代进行作图，并且仅需要90次PCR扩增。已确定同线标记之间的遗传距离，以确保基因组的完全覆盖，并允许连锁数据的插值。因此，大多数突变应在不到一个月的时间内绘制到1-5个图单位内，这一分辨率水平足以启动基于图的克隆工作。该系统也将有助于在全基因组水平上分析重组，并适用于其他完美的真菌时，合适的标记。(c)2006年爱思唯尔公司All rights reserved.
Forward genetic analysis is the most broadly applicable approach to discern gene functions. However, for some organisms like the filamentous ascomycete Neurospora crassa, genetic mapping frequently represents a limiting step in forward genetic approaches. We describe an efficient method for genetic mapping in N. crassa that makes use of a modified bulked segregant analysis and PCR-based molecular markers. This method enables mapping with progeny from a single cross and requires only 90 PCR amplifications. Genetic distances between syntenic markers have been determined to ensure complete coverage of the genome and to allow interpolation of linkage data. As a result, Most Mutations should be mapped in less than one month to within 1-5 map units, a level of resolution sufficient to initiate map-based cloning efforts. This system also will facilitate analyses of recombination at a genome-wide level and is applicable to other perfect fungi when suitable markers are available. (c) 2006 Elsevier Inc. All rights reserved.