Presence of immunoreactive corticotropin-releasing factor in rat testis.
Presence of immunoreactive corticotropin-releasing factor in rat testis.
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DOI：
10.1210/endo-122-2-759
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发表时间：
1988-02
期刊：
影响因子：
4.8
通讯作者：
Doug-Jin Yoon;C. Sklar;R. David
中科院分区：
文献类型：
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作者：
Doug-Jin Yoon;C. Sklar;R. David
我们试图通过放射免疫分析和免疫细胞化学技术证明免疫反应(Ir)CRF在大鼠睾丸中的存在。对27,000×g成年大鼠睾丸磷酸盐缓冲液上清液进行放射免疫分析，结果显示irCRF含量很高(51-74 ng/g睾丸)，与人工合成大鼠CRF制备的标准曲线有明显的平行关系。相比之下，irCRF的外周血液水平极低(<0.05 ng/ml)。免疫细胞化学研究表明，在正常成年大鼠睾丸的间质细胞和生殖细胞中，irCRF呈强烈的特异性染色。附睾精子染色也呈阳性。睾丸irCRF随年龄增长波动明显。10日龄、20日龄、60日龄和90日龄大鼠睾丸的平均+/-SD分别为41.6+/-4.7、8.7+/-0.2、55.5+/-3.3和71.3+/-3.4 ng/g睾丸(P<0.01)。成年大鼠腹部移位睾丸(9.6+/-1.3 ng/g睾丸)和切除脑垂体(16.7+/-1.6 ng/g睾丸)后，其水平显著降低。然而，半去势和单侧隐睾术都不影响对侧睾丸中irCRF的水平。这些数据表明局部存在irCRF的产生，这可能在调节睾丸和附睾的间质细胞功能和精子成熟中发挥作用。
We attempted to demonstrate the presence of immunoreactive (ir) CRF in rat testis by RIA, and by an immunocytochemical technique. The RIA was performed on 27,000 x g supernatants of phosphate buffer extracts of adult rat testes, and revealed a high concentration of irCRF (51 - 74 ng/g testis), with a clear parallelism to the standard curve prepared with synthetic rat CRF. In contrast, the peripheral blood level of irCRF was extremely low (less than 0.05 ng/ml). Immunocytochemical studies of irCRF revealed strong specific staining in the Leydig cells and germ cells in normal adult rat testis. Epididymal spermatozoa also stained positive. Testicular irCRF fluctuated significantly with age. The levels (mean +/- SD) assayed in 10, 20, 60 and 90-day-old rats were 41.6 +/- 4.7, 8.7 +/- 0.2, 55.5 +/- 3.3 and 71.3 +/- 3.4 ng/g testis, respectively (P less than 0.01). The level was drastically reduced after abdominal translocation of a testis (9.6 +/- 1.3 ng/g testis), and after hypophysectomy (16.7 +/- 1.6 ng/g testis) in adult rats. However, neither hemicastration nor unilateral cryptorchidism influenced the irCRF levels in the contralateral testis. These data suggest a local production of irCRF which may play a role in regulation of Leydig cell function and sperm maturation in testis and epididymis.