Enhancement of radiotherapy by ceria nanoparticles modified with neogambogic acid in breast cancer cells.
Enhancement of radiotherapy by ceria nanoparticles modified with neogambogic acid in breast cancer cells.
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新藤黄酸修饰的二氧化铈纳米粒子增强乳腺癌细胞的放射治疗
DOI：
10.2147/ijn.s82980
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发表时间：
2015
影响因子：
8
通讯作者：
Zhang HQ
中科院分区：
文献类型：
--
作者：
Chen F;Zhang XH;Hu XD;Zhang W;Lou ZC;Xie LH;Liu PD;Zhang HQ
放射治疗是癌症治疗的主要策略之一，但具有重大挑战，例如癌细胞抗性和对正常组织的辐射损伤。选择性增加癌细胞对辐射的敏感性的放射增敏剂可以增强放射治疗的有效性。我们在这里报告的发展，一种新的放射增敏剂组成的单分散的二氧化铈纳米粒子（CNPs）覆盖的抗癌药物新藤黄酸（NGA-CNPs）。这些与MCF-7乳腺癌细胞中的辐射结合使用，并评估了这种联合治疗方法的疗效和作用机制。NGA-CNP增强了辐射的毒性作用，导致比单独使用的任一种治疗更高的细胞死亡率，并诱导自噬的激活和G2/M期的细胞周期停滞，而用NGA或CNP预处理并没有改善辐射诱导的癌细胞死亡率。然而，与其他纳米材料不同，NGA-CNP降低了内源性和辐射诱导的活性氧的形成。这些结果表明，连续使用NGA-CNP可以通过降低杀死癌细胞所需的辐射剂量来增加乳腺癌治疗中放射治疗的有效性，从而最大限度地减少对健康邻近组织的附带损伤。
Radiotherapy is one of the main strategies for cancer treatment but has significant challenges, such as cancer cell resistance and radiation damage to normal tissue. Radiosensitizers that selectively increase the susceptibility of cancer cells to radiation can enhance the effectiveness of radiotherapy. We report here the development of a novel radiosensitizer consisting of monodispersed ceria nanoparticles (CNPs) covered with the anticancer drug neogambogic acid (NGA-CNPs). These were used in conjunction with radiation in MCF-7 breast cancer cells, and the efficacy and mechanisms of action of this combined treatment approach were evaluated. NGA-CNPs potentiated the toxic effects of radiation, leading to a higher rate of cell death than either treatment used alone and inducing the activation of autophagy and cell cycle arrest at the G2/M phase, while pretreatment with NGA or CNPs did not improve the rate of radiation-induced cancer cells death. However, NGA-CNPs decreased both endogenous and radiation-induced reactive oxygen species formation, unlike other nanomaterials. These results suggest that the adjunctive use of NGA-CNPs can increase the effectiveness of radiotherapy in breast cancer treatment by lowering the radiation doses required to kill cancer cells and thereby minimizing collateral damage to healthy adjacent tissue.