Streptomycin Sodium Azide and Merthiolate as Bacteriostatic Agents for Use with the Treponema pallidum Immobilization Test*
Streptomycin Sodium Azide and Merthiolate as Bacteriostatic Agents for Use with the Treponema pallidum Immobilization Test*
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链霉素叠氮化钠和硫柳汞作为抑菌剂用于梅毒螺旋体固定试验*
DOI：
10.1136/sti.35.1.53
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发表时间：
1959
期刊：
影响因子：
--
通讯作者：
G. R. Cannefax
中科院分区：
文献类型：
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作者：
G. R. Cannefax
当用梅毒螺旋体固定（TPI）测试来测试要测试的血清样本时，与用传统的梅毒血清学测试测试的血清样本的运输或保存相关的细菌污染问题会增加。细菌污染会影响读取测试的简便性和准确性，并且某些污染物的代谢或体细胞产物会产生非特异性固定。该报告证实了 Ledbetter 和 Cumming (1954) 报道的链霉素作为抑菌剂的价值，并且还表明叠氮化钠和硫柳汞是用于 TPI 测试的令人满意的抑菌剂。 Ledbetter 和 Cumming 使用浓度为 100 ug./ml 的链霉素。防止提交测试的受细菌污染的样本进一步生长。他们得出的结论是浓度为 100 微克/毫升。对梅毒螺旋体的活力或残留补体反应没有有害影响。他们没有报告 100 tg./ml 的效果。集中于测试的灵敏度。该实验室的研究表明，100 克/毫升。不会显着降低测试灵敏度。 11次定量测试，血清含量为100μLg./ml。给出了几何平均值 50%。固定滴度为 1: 53，而没有链霉素时，几何平均滴度为 1: 74。这一小系列的比较测试表明，在 Ledbetter 和 Cumming 使用的浓度下，链霉素不会显着降低测试的灵敏度。在
The problems of bacterial contamination associated with the shipment or preservation of serum specimens to be tested with the conventional sero-logic tests for syphilis are increased when such specimens are to be tested with the Treponema pallidum Immobilization (TPI) test. Bacterial contamination interferes with the ease and accuracy of reading the test and the metabolic or somatic products of certain contaminants produce non-specific immobilization. This report confirms the value of streptomycin as a bacteriostat, as reported by Ledbetter and Cumming (1954), and also shows that sodium azide and merthiolate are satisfactory bacteriostats for use with the TPI test. Ledbetter and Cumming employed streptomycin at a concentration of 100 ug./ml. to prevent further growth in bacterially contaminated specimens submitted for testing. They concluded that a concentration of 100,ug./ml. didnot have a harmful effect on the viability of Treponema pallidum or the residual complement reaction. They did not report on the effect of 100 ,tg./ml. concentration on the sensitivity of the test. Studies in this laboratory indicate that 100 ,g./ml. does not lower test sensitivity significantly. Eleven quantitative tests with sera containing 100 jLg./ml. gave a geometric mean 50 per cent. immobilization titre of 1: 53, while without streptomycin the geometric mean titre was 1: 74. This small series of comparative tests indicates that streptomycin, in the concentration employed by Ledbetter and Cumming, does not reduce significantly the sensitivity of the test. In