BORRELIA-BURGDORFERI SWIMS WITH A PLANAR WAVE-FORM SIMILAR TO THAT OF EUKARYOTIC FLAGELLA
BORRELIA-BURGDORFERI SWIMS WITH A PLANAR WAVE-FORM SIMILAR TO THAT OF EUKARYOTIC FLAGELLA
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DOI：
10.1073/pnas.91.8.3433
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发表时间：
1994-04-12
影响因子：
11.1
通讯作者：
KREILING, JA
中科院分区：
文献类型：
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作者：
GOLDSTEIN, SF;CHARON, NW;KREILING, JA
伯氏疏螺旋体是一种游动螺旋体，在细胞圆柱体的两端附近附着有多个内部周质鞭毛（PF）;这些PF在细胞中心重叠。我们分析了野生型和PF缺陷突变体的形状和运动，使用显微摄影和视频显微镜。我们发现游动细胞类似于真核生物鞭毛的动态运动。与螺旋形螺旋体相反，螺旋形螺旋体传播螺旋波，平移B。Burgdorferi以偶尔轴向扭曲的平面波形游动;波具有0.85 μ m的峰-峰振幅和3.19 μ m的波长。平面波在前端开始全尺寸，并向细胞的后端传播。同时，这些波逆时针旋转，从后端沿着细胞轴。在非翻译细胞中，波传播停止。非翻译细胞的波形与翻译形式相似，或者细胞变得明显扭曲。Sadziene等人分离的PF缺陷突变体的细胞[Sadziene，A.，托马斯，D. D、Bundoc，V.G.，Holt，S. C. & Barclay，A. G.（1991）J. Clin. Invest. 88，82-92]被发现是相对直。结果表明，B. burgdorferi是由细胞体和PF之间的相互作用决定的。此外，据信来自细胞相对端的PF彼此相互作用，使得在显著扭曲的非平移形式期间，来自相对端的PF围绕彼此沿相反方向旋转，导致细胞弯曲。
Borrelia burgdorferi is a motile spirochete with multiple internal periplasmic flagella (PFs) attached near each end of the cell cylinder; these PFs overlap in the cell center. We analyzed the shape and motion of wild type and PF-deficient mutants using both photomicrography and video microscopy. We found that swimming cells resembled the dynamic movements of eukaryotic flagella. In contrast to helically shaped spirochetes, which propagate spiral waves, translating B. burgdorferi swam with a planar waveform with occasional axial twists; waves had a peak-to-peak amplitude of 0.85 mum and a wavelength of 3.19 mum. Planar waves began full-sized at the anterior end and propagated toward the back end of the cell. Concomitantly, these waves gyrated counter-clockwise as viewed from the posterior end along the cell axis. In nontranslating cells, wave propagation ceased. Either the waveform of nontranslating cells resembled the translating form, or the cells became markedly contorted. Cells of the PF-deficient mutant isolated by Sadziene et al. [Sadziene, A., Thomas, D. D., Bundoc, V. G., Holt, S. C. & Barbour, A. G. (1991) J. Clin. Invest. 88, 82-92] were found to be relatively straight. The results suggest that the shape of B. burgdorferi is dictated by interactions between the cell body and the PFs. In addition, the PFs from opposite ends of the cell are believed to interact with one another so that during the markedly distorted nontranslational form, the PFs from opposite ends rotate in opposing directions around one another, causing the cell to bend.