Effects of stage of the menstrual cycle and gonadotrophins on luteinization of rhesus monkey granulosa cells in culture.
Effects of stage of the menstrual cycle and gonadotrophins on luteinization of rhesus monkey granulosa cells in culture.
复制标题
月经周期阶段和促性腺激素对培养的恒河猴颗粒细胞黄素化的影响。
DOI：
10.1210/endo-87-1-49
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发表时间：
1970
期刊：
影响因子：
4.8
通讯作者：
C. Channing
中科院分区：
文献类型：
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作者：
C. Channing
在月经周期第 12-14 天从恒河猴排卵前大卵泡中收获颗粒细胞，在培养物中黄素化；在培养长达 6 天的时间里，它们的大小和细胞质与细胞核的比例增加，积累了大量的细胞质颗粒和脂滴，并分泌高水平的孕激素（90-200 皮克/细胞/天）。此后孕激素分泌率和细胞数量逐渐下降。从 1 毫米小卵泡中收获的细胞没有黄素化。从周期的第 7 天到第 11 天从猴子的中等大小的卵泡中收获的细胞部分黄素化或根本不黄素化。在周期的所有阶段，将人 LH 或 FSH 单独添加到从猴子 1 mm 卵泡中收获的颗粒细胞培养物中不会诱导黄体化；然而，LH 和 FSH 的混合物确实在这些培养物中诱导了黄体化。相比之下，在周期第 7 至 11 天从猴子中等大小的卵泡中收获的颗粒细胞培养物中单独添加人 LH 或 FSH 会诱导形态变化。
Granulosa cells harvested from large preovulatory follicles of rhesus monkeys at days 12–14 of the menstrual cycle luteinized in culture; they increased in size and cytoplasmic-nuclear ratio, accumulated numerous cytoplasmic granules and lipid droplets and secreted elevated levels of progestins (90–200 pg/cell/day) up to 6 days in culture. Progestin secretion rates and cell numbers decreased gradually after this time. Cells harvested from small 1 mm follicles did not luteinize. Cells harvested from intermediate-sized follicles of monkeys from day 7 t o 11 of the cycle luteinized partially or not at all. Addition of human LH or FSH alone to cultures of granulosa cells harvested from 1 mm follicles of monkeys at all stages of the cycle did not induce luteinization; however, a mixture of LH and FSH did induce luteinization in these cultures. In contrast, addition of human LH or FSH alone to cultures of granulosa cells harvested from medium-sized follicles of monkeys from day 7 to 11 of the cycle induced morp...