Sequences analyses and expression profiles in tissues and embryos of Japanese flounder (Paralichthys olivaceus) PRDM1
Sequences analyses and expression profiles in tissues and embryos of Japanese flounder (Paralichthys olivaceus) PRDM1
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DOI：
10.1007/s10695-015-0152-8
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发表时间：
2016-04-01
影响因子：
2.9
通讯作者：
Wang, Xubo
中科院分区：
文献类型：
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作者：
Liu, Conghui;Liu, Wei;Wang, Xubo
PRDM 1（PRDI-BF 1-RIZ 1 homologous domain containing 1）是一种多效调节因子，参与多种生物学过程。它含有一个PR（PRDI-BF 1-RIZ 1同源）结构域蛋白和五个锌指。在本研究中，从牙鲆中鉴定出一个编码prdm 1同源物及其5'调控区的基因（Po-prdm 1）。实时荧光定量PCR（RT-qPCR）和原位杂交（ISH）结果显示，Po-prdm 1在胚胎早期和尾芽期高表达，在胚胎中期达到高峰，而组织中的RT-qPCR和ISH结果表明，Po-prdm 1转录本在所有组织中都普遍存在，这表明其在多个过程中的多效性功能。对性腺组织的原位杂交显示，转录本定位于卵巢卵母细胞的细胞核和细胞质中，但仅定位于精原细胞中，而未定位于睾丸的精母细胞中。本研究分析了Po-prdm 1转录因子结合位点及其在脊椎动物中的保守结合区。综合结果表明，Po-PRDM 1在硬骨鱼和哺乳动物中具有保守的功能。
PRDM1 (PRDI-BF1-RIZ1 homologous domain containing 1) appears to be a pleiotropic regulatory factor in various processes. It contains a PR (PRDI-BF1-RIZ1 homologous) domain protein and five zinc fingers. In the present study, a gene coding the homolog of prdm1 and the 5' regulatory region of prdm1 was identified from the Paralichthys olivaceus (denoted Po-prdm1). Results of real-time quantitative polymerase chain reaction amplification (RT-qPCR) and in situ hybridization (ISH) in embryos revealed that Po-prdm1 was highly expressed between the early gastrula and tail bud stages, with its expression peaking in the mid-gastrula stage, whereas the results of RT-qPCR and ISH in tissues demonstrated that Po-prdm1 transcripts were ubiquitously detected in all tissues, which indicates its pleiotropic function in multiple processes. ISH of gonadal tissues revealed that the transcripts were located in the nucleus and cytoplasm of the oocytes in the ovaries but only in the spermatogonia and not in the spermatocytes in the testes. The Po-prdm1 transcription factor binding sites and their conserved binding region among vertebrates were analyzed in this study. The combined results suggest that Po-PRDM1 has a conserved function in teleosts and mammals.