The effects of prostaglandin E1 on megakaryocyte proliferation in vitro.
The effects of prostaglandin E1 on megakaryocyte proliferation in vitro.
复制标题
前列腺素E1对体外巨核细胞增殖的影响。
DOI：
10.1007/978-1-4684-5571-7_24
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发表时间：
1988
影响因子：
--
通讯作者：
Hou,XP
中科院分区：
文献类型：
--
作者：
Cooper,GW;Hou,XP
在血浆凝块培养系统中孵育4天后，在含有10%血清和10%血浆或20%血浆和24×106个/毫升血小板提取液的培养液中，前列腺素E_1浓度为560 nM时，巨核细胞集落/凝块和巨核细胞/凝块数量均显著高于对照组。在含血清的培养中，在整个14-560 nm范围内，PGE1浓度与巨核细胞集落/凝块和巨核细胞/凝块的数量之间也显示出线性的剂量-反应关系。然而，在只含有20%大鼠血浆的培养中，PGE1似乎不能刺激巨核细胞。
A concentration of 560 nM Prostaglandin E1(PGE1), in a medium containing either 10% serum and 10% plasma, or 20% plasma and a platelet extract prepared from 24 × 106platelets/ml, caused after four days of incubation in a plasma clot culture system, highly significant increases over the controls in both the number of megakaryocyte colonies/clot and megakaryocytes/clot. A linear dose-response relationship over the entire 14–560 nM range was also demonstrated in cultures containing serum, between the concentration of PGE1in each culture, and the number of megakaryocyte colonies/clot and megakaryocytes/clot. However, PGE1did not appear to stimulate megakaryocytes in cultures containing only 20% rat plasma.