ESTABLISHMENT OF HUMAN ENDOMETRIAL ADENOCARCINOMA CELL-LINE CONTAINING ESTRADIOL-17-BETA AND PROGESTERONE RECEPTORS
ESTABLISHMENT OF HUMAN ENDOMETRIAL ADENOCARCINOMA CELL-LINE CONTAINING ESTRADIOL-17-BETA AND PROGESTERONE RECEPTORS
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DOI：
10.1016/0090-8258(84)90212-9
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发表时间：
1984-01-01
影响因子：
4.7
通讯作者：
ISHIKAWA, H
中科院分区：
文献类型：
--
作者：
ISHIWATA, I;ISHIWATA, C;ISHIKAWA, H
命名为HHUA的细胞系是从一名54岁女性的分化良好的子宫内膜腺癌（1级）建立的。细胞生长良好，连续22个月。并继代培养了56次以上。细胞呈梭形或多角形，呈多层结构，无接触抑制。染色体数目变异较大，表现为非整倍体。众数为46，未发现标记染色体。将细胞移植于BALB/C裸鼠皮下，并产生肿瘤，解释为腺癌。细胞含有雌二醇-17 β。和孕酮受体。雌二醇-17 β的给药成功地耗尽了细胞质雌激素受体，并增加了孕酮受体水平。
The cell line designated HHUA was established from a well-differentiated endometrial adenocarcinoma (grade 1) of a 54-yr-old female. The cell line grew well without interruption for 22 mo. and was subcultivated more than 56 times. The cells were spindle or polygonal in shape, formed multilayers, and did not show contact inhibition. The chromosome number varied widely and showed aneuploidy. The modal number was 46, and no marker chromosome was identified. The cells were transplanted into the subcutis of BALB/C nude mice, and produced tumors which were interpreted as adenocarcinoma. The cells contained estradiol-17.beta. and progesterone receptors. Administration of estradiol-17.beta. successfully depleted cytoplasmic estrogen receptors and brought about increased progesterone receptor levels.