CHARACTERIZATION OF THE UPP-GENE ENCODING URACIL PHOSPHORIBOSYLTRANSFERASE OF ESCHERICHIA-COLI K12
CHARACTERIZATION OF THE UPP-GENE ENCODING URACIL PHOSPHORIBOSYLTRANSFERASE OF ESCHERICHIA-COLI K12
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DOI：
10.1111/j.1432-1033.1992.tb16604.x
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发表时间：
1992-02-15
期刊：
影响因子：
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通讯作者：
MYGIND, B
中科院分区：
文献类型：
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作者：
ANDERSEN, PS;SMITH, JM;MYGIND, B
将编码尿嘧啶磷酸核糖基转移酶的upp基因与purMN操纵子一起沿着亚克隆在5-kb EcoRI限制性片段上。 通过互补、缺失和小细胞分析的组合，upp基因位于purMN操纵子附近，并从purMN操纵子分化转录。 所有三种基因产物都可以在小细胞提取物中鉴定。 克隆的upp基因在尿嘧啶饥饿时表现出升高的表达。 测定了该基因的核苷酸序列和转录起始位点。 该序列产生624个核苷酸的开放阅读框，编码22.5 kDa的蛋白质，其与先前确定的纯化酶的亚基M（r）一致。 已经鉴定了一个推定的5-磷酸核糖基-α-1-二磷酸（PRPP）结合位点，其与酵母尿嘧啶磷酸核糖基转移酶的PRPP结合位点相似。
The upp gene coding for uracil phosphoribosyltransferase was subcloned on a 5-kb EcoRI restriction fragment along with the purMN operon. By a combination of complementation, deletion and minicell analyses, the upp gene was located adjacent to and divergently transcribed from the purMN operon. All three gene products could be identified in minicell extracts. The cloned upp gene shows an elevated expression upon uracil starvation. The nucleotide sequence and transcription start of the gene were determined. The sequence yields an open reading frame of 624 nucleotides encoding a protein of 22.5 kDa which is in agreement with the previously determined subunit M(r) of the purified enzyme. A putative 5-phosphoribosyl-alpha-1-diphosphate (PRPP) binding site has been identified which is similar to the PRPP binding site of the yeast uracil phosphoribosyltransferase.