Limited modulation of the mitogen-activated protein kinase pathway by cyclic AMP in rat parotid acinar cells.
Limited modulation of the mitogen-activated protein kinase pathway by cyclic AMP in rat parotid acinar cells.
复制标题
大鼠腮腺腺泡细胞中环 AMP 对丝裂原激活蛋白激酶途径的有限调节。
DOI：
10.1006/bbrc.1994.1993
复制
发表时间：
1994
影响因子：
3.1
通讯作者：
Humphreys-Beher,MG
中科院分区：
文献类型：
--
作者：
Purushotham,KR;Wang,PL;Humphreys-Beher,MG
Treatment of rat parotid acinar cells,in vitro, with agents that elevate intracellular cAMP had only limited impact on the ability of EGF to subsequently stimulate MAP kinase activity or phosphorylation. A time course of cAMP accumulation followingin vivoadministration of isoproterenol showed the greatest level of cAMP 15 min following the primary injection. Over a 72 hr injection regimen, agonist-stimulated cAMP levels were gradually reduced to control levels while cAMP-dependent protein kinase A (PKA) activity in immunoprecipitates of Raf-1 or MAP-kinase remained elevated. Raf-1 did not undergo phosphorylation following incubation with the catalytic subunit of PKA, suggesting that in normal rat acinar cells proliferation induced by isoproterenol or EGF involves the p21ras-Raf-MAP-kinase signaling cascade despite the presence of cAMP.
