Magnetic resonance fluoroscopy allows targeted delivery of mesenchymal stem cells to infarct borders in swine
Magnetic resonance fluoroscopy allows targeted delivery of mesenchymal stem cells to infarct borders in swine
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DOI：
10.1161/01.cir.0000095790.28368.f9
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发表时间：
2003-12-09
期刊：
影响因子：
37.8
通讯作者：
Lederman, RJ
中科院分区：
文献类型：
--
作者：
Dick, AJ;Guttman, MA;Lederman, RJ
传递间充质干细胞（MSCs）的局部环境可能影响其最终表型。磁共振透视有可能引导心肌内骨髓间充质干细胞注射到理想的目标，如梗死组织和正常组织之间的边界。我们测试了以下能力：(1)识别梗死，(2)将注射导管导航到预先选择的目标，(3)即使在新梗死中也能安全注射，以及(4)立即确认注射成功。方法与结果：定制1.5 t MRI扫描仪用于介入治疗，具有快速成像，导管通道独立显色，多层3D渲染，仅导管观看模式和梗死增强成像。将MRI接收线圈置入导尿管和注射针中。这些装置进行了加热测试，并用于靶向MSC递送。在梗死猪中，通过磁共振透视瞄准心肌。梗死增强成像包括静脉注射钆后的饱和准备MRI和壁运动。用铁荧光颗粒对猪间充质干细胞进行mri标记。导管导航和多个细胞注射完全在MR透视下进行，速度为8帧/秒，体素为1.7X3.3X8-mm。梗死增强MR透视可以很好地描绘梗死边界。所有的注射都安全并成功地递送到预定的目标，包括梗死边界。铁荧光颗粒标记的间充质干细胞在体内和死后都很容易观察到。结论：利用磁共振导管给药系统对近期心肌梗死边界进行潜在再生细胞治疗是可行的。磁共振透视允许可视化导管导航，心肌功能，梗死边界，和标记细胞注射后。
Background-The local environment of delivered mesenchymal stem cells (MSCs) may affect their ultimate phenotype. MR fluoroscopy has the potential to guide intramyocardial MSC injection to desirable targets, such as the border between infarcted and normal tissue. We tested the ability to (1) identify infarcts, (2) navigate injection catheters to preselected targets, (3) inject safely even into fresh infarcts, and (4) confirm injection success immediately.Methods and Results-A 1.5-T MRI scanner was customized for interventional use, with rapid imaging, independent color highlighting of catheter channels, multiple-slice 3D rendering, catheter-only viewing mode, and infarct-enhanced imaging. MRI receiver coils were incorporated into guiding catheters and injection needles. These devices were tested for heating and used for targeted MSC delivery. In infarcted pigs, myocardium was targeted by MR fluoroscopy. Infarct-enhanced imaging included both saturation preparation MRI after intravenous gadolinium and wall motion. Porcine MSCs were MRI-labeled with iron-fluorescent particles. Catheter navigation and multiple cell injections were performed entirely with MR fluoroscopy at 8 frames/s with 1.7X3.3X8-mm voxels. Infarct-enhanced MR fluoroscopy permitted excellent delineation of infarct borders. All injections were safely and successfully delivered to their preselected targets, including infarct borders. Iron-fluorescent particle-labeled MSCs were readily visible on delivery in vivo and post mortem.Conclusions-Precise targeted delivery of potentially regenerative cellular treatments to recent myocardial infarction borders is feasible with an MR catheter delivery system. MR fluoroscopy permits visualization of catheter navigation, myocardial function, infarct borders, and labeled cells after injection.