The Initial Stages of Template-Controlled CaCO3 Formation Revealed by Cryo-TEM
The Initial Stages of Template-Controlled CaCO3 Formation Revealed by Cryo-TEM
复制标题
DOI：
10.1126/science.1169434
复制
发表时间：
2009-03-13
期刊：
影响因子：
56.9
通讯作者：
Sommerdijk, Nico A. J. M.
中科院分区：
文献类型：
--
作者：
Pouget, Emilie M.;Bomans, Paul H. H.;Sommerdijk, Nico A. J. M.
生物碳酸钙形成贝壳、耳石和许多海洋骨骼的无机成分，其形成由有序的大分子模板指导。经典的成核理论认为晶体的形成是由溶液中离子聚集形成的临界核发生的。使用冷冻透射电子显微镜，我们发现，模板导向碳酸钙的形成开始与核前簇的形成。它们的聚集导致溶液中无定形纳米颗粒的成核。这些纳米颗粒在模板处组装，并且在达到临界尺寸后，发展动态结晶域，其中一个由模板选择性地稳定。我们的研究结果对生物和合成系统中模板导向的矿物形成具有影响。
Biogenic calcium carbonate forms the inorganic component of seashells, otoliths, and many marine skeletons, and its formation is directed by an ordered template of macromolecules. Classical nucleation theory considers crystal formation to occur from a critical nucleus formed by the assembly of ions from solution. Using cryotransmission electron microscopy, we found that template-directed calcium carbonate formation starts with the formation of prenucleation clusters. Their aggregation leads to the nucleation of amorphous nanoparticles in solution. These nanoparticles assemble at the template and, after reaching a critical size, develop dynamic crystalline domains, one of which is selectively stabilized by the template. Our findings have implications for template-directed mineral formation in biological as well as in synthetic systems.