Discovery of novel plasma proteins as biomarkers for the development of incisional hernias after midline incision in patients with colorectal cancer: The ColoCare study
Discovery of novel plasma proteins as biomarkers for the development of incisional hernias after midline incision in patients with colorectal cancer: The ColoCare study
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DOI：
10.1016/j.surg.2016.08.025
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发表时间：
2017-03-01
期刊：
影响因子：
3.8
通讯作者：
Ulrich, Cornelia M.
中科院分区：
文献类型：
--
作者：
Boehm, Juergen;Pianka, Frank;Ulrich, Cornelia M.
背景资料。腹壁切口疝是腹部手术后最常见的长期并发症。在新诊断的结直肠癌患者中，我们筛选了术前血浆蛋白质组，以探索发生切口疝的预测标记物。我们使用了72例新诊断的结直肠癌患者的术前血浆样本，这些患者在2010-2013年间接受了正中切口肿瘤切除。根据性别、年龄和体重指数，共有21名发生切口疝的患者与51名随访至少18个月的无切口疝的患者进行配对。为了评估切口疝气风险的预测标记物，我们使用经过充分验证的抗体微阵列测试来筛选血浆蛋白质组中的2000种不同的蛋白质。配对t检验用于比较病例和对照组之间的蛋白质水平。基因集浓缩分析(基因本体学和京都基因和基因组百科全书)用来检验两组之间信号通路的差异。蛋白质组筛查发现，与对照组相比，疝气组血浆水平升高或降低的蛋白质有25种(标称P值
Background. Ventral incisional hernia is the most common long-term complication after an abdominal operation. Among newly diagnosed colorectal cancer patients, we screened the preoperative plasma proteome to explore predictive markers for the development of an incisional hernia.Methods. We utilized preoperative plasma samples of 72 newly diagnosed colorectal cancer patients who underwent midline incision for tumor resection between 2010 and 2013. A total of 21 patients with incisional hernia occurrence were matched with 51 patients with at least 18 months follow-up without an incisional hernia by sex, age, and body mass index. To assess predictive markers of incisional hernia risk, we screened the plasma proteome for > 2,000 distinct proteins using a well-validated antibody microarray test. Paired t tests were used to compare protein levels between cases and controls. A gene-set enrichment analysis (Gene Ontology and Kyoto Encyclopedia of Genes and Genomes) was applied to test for differences in signaling pathways between the 2 groups.Results. The proteome screen identified 25 proteins that showed elevated or reduced plasma levels in the hernia group compared to the control group (nominal P values