At least three subdomains of v-erbA are involved in its silencing function.
At least three subdomains of v-erbA are involved in its silencing function.
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v-erbA 的至少三个子结构域参与其沉默功能。
DOI：
10.1210/mend.11.3.9903
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发表时间：
1997
影响因子：
--
通讯作者：
M. Muller
中科院分区：
文献类型：
--
作者：
K. Busch;B. Martin;A. Baniahmad;R. Renkawitz;M. Muller
Several members of the thyroid hormone receptor (TR) family are able to switch from a transcriptional repressor to a transcriptional activator upon binding of their ligand. The oncogene v-erbA is a variant form of the TR unable to bind hormone and thus acts as a constitutive repressor. We demonstrate, using fusion proteins between the DNA-binding domain of the yeast factor GAL4 and the silencing domains of v-erbA and TR beta, that point mutations in three different regions severely affect their repression function. Furthermore, the three regions, each as an inactive fusion protein with the GAL4 DNA-binding domain, restore silencing activity when assembled on the same promoter. These observations define at least three silencing subdomains, SSD1-SSD3, which are involved in the silencing function of v-erbA. We propose a model in which full silencing activity is brought about by the combined interaction of each silencing subdomain with corepressors and/or basal transcription factors.
影响因子：
10.5
作者：
Silver,PA;Chiang,A;Sadler,I
通讯作者：
Sadler,I