The Promoter Toolbox for Recombinant Gene Expression in Trichoderma reesei.
The Promoter Toolbox for Recombinant Gene Expression in Trichoderma reesei.
复制标题
trichoderma Reesei中重组基因表达的启动子工具箱。
DOI：
10.3389/fbioe.2018.00135
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发表时间：
2018
影响因子：
5.7
通讯作者：
Seiboth B
中科院分区：
文献类型：
--
作者：
Fitz E;Wanka F;Seiboth B
子囊菌里氏木霉（Trichoderma reesei）是纤维素酶和木聚糖酶的主要产酶真菌之一。其酶应用于食品、饲料和纺织工业或生物燃料和生物精炼工业中的木质纤维素水解。在过去的几年里，扩大T.有利于里氏杆菌基因工程和提高异源蛋白产量的生长。调控关键基因表达的一个重要工具是启动子，启动和控制其转录。迄今为止，最常用的T. Reesei是主要纤维二糖水解酶Cel7A的强诱导型启动子。除此之外，还有许多来自其他纤维素酶和木聚糖酶编码基因的替代诱导型启动子和一些组成型启动子。随着基因组学和转录组学的发展，具有不同表达强度的组成型和可调启动子的鉴定变得简单。在这篇综述中，我们将讨论启动子领域的新发展，并比较它们的优缺点。合成表达系统构成了控制基因表达和构建复杂基因回路的新选择。因此，我们将讨论启动子的共同结构特征，并描述启动子工程和启动子合成设计的选择。良好表征的基因表达控制工具的可用性对于分析基因功能、检测基因网络中的瓶颈和提高生物技术应用的产量至关重要。
The ascomycete Trichoderma reesei is one of the main fungal producers of cellulases and xylanases based on its high production capacity. Its enzymes are applied in food, feed, and textile industry or in lignocellulose hydrolysis in biofuel and biorefinery industry. Over the last years, the demand to expand the molecular toolbox for T. reesei to facilitate genetic engineering and improve the production of heterologous proteins grew. An important instrument to modify the expression of key genes are promoters to initiate and control their transcription. To date, the most commonly used promoter for T. reesei is the strong inducible promoter of the main cellobiohydrolase cel7a. Beside this one, there is a number of alternative inducible promoters derived from other cellulase- and xylanase encoding genes and a few constitutive promoters. With the advances in genomics and transcriptomics the identification of new constitutive and tunable promoters with different expression strength was simplified. In this review, we will discuss new developments in the field of promoters and compare their advantages and disadvantages. Synthetic expression systems constitute a new option to control gene expression and build up complex gene circuits. Therefore, we will address common structural features of promoters and describe options for promoter engineering and synthetic design of promoters. The availability of well-characterized gene expression control tools is essential for the analysis of gene function, detection of bottlenecks in gene networks and yield increase for biotechnology applications.
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DOI：
10.1016/j.bbagrm.2012.02.014
发表时间：
2012-07
影响因子：
4.7
作者：
Chatterjee, Raghunath;Vinson, Charles
通讯作者：
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影响因子：
6.4
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DOI：
10.1002/yea.3292
发表时间：
2018-03
期刊：
Yeast (Chichester, England)
影响因子：
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通讯作者：
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影响因子：
6.3
作者：
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影响因子：
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