Effects of the ionic liquid 1-hexyl-3-methylimidazolium bromide on root gravitropism in Arabidopsis seedlings.
Effects of the ionic liquid 1-hexyl-3-methylimidazolium bromide on root gravitropism in Arabidopsis seedlings.
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DOI：
10.1016/j.ecoenv.2015.11.038
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发表时间：
2016-03
影响因子：
6.8
通讯作者：
Liang Zhang;Tianqi Wang;F. Zheng;Lingyu Ma;Jingyuan Li
中科院分区：
文献类型：
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作者：
Liang Zhang;Tianqi Wang;F. Zheng;Lingyu Ma;Jingyuan Li
近年来，离子液体的毒性效应越来越受到人们的关注。然而，关于离子液体对生物体向性的毒性作用的知识仍然非常有限。本研究以拟南芥幼苗为材料，研究了溴化1-己基-3-甲基咪唑[C6 mim]Br对根系向地性的影响。结果表明，随着IL浓度的增加，根系生长和重力反应受到显著抑制。与对照相比，[C6 mim]Br处理影响了根冠淀粉体的数量和分布格局。用DR 5 rev：：VENUS标记的生长素分布在IL处理的幼苗中被改变。生长素外排载体PIN 2和PIN 3的信号强度和基因表达在IL胁迫下明显降低。此外，作为响应重力刺激的结果，对照根尖中的不对称DR 5信号被IL处理削弱。在IL处理的幼苗中，沿根的下胁沿着的主要PIN 2信号和柱细胞中的PIN 3极化也显著降低。我们的研究结果表明，离子液体[C6 mim]Br影响淀粉体的数量和分布，并干扰部署的PIN 2和PIN 3，从而削弱生长素流响应重力刺激，导致缺乏根向重力性inclusionopsis幼苗。
The toxic effects of ionic liquids (ILs) have attracted increasing attention in recent years. However, the knowledge about the toxic effects of ILs on tropism in organisms remains quite limited. In this study, the effects of 1-hexyl-3-methylimidazolium bromide [C6mim]Br on root gravitropism were evaluated usingArabidopsisseedlings. Our results showed that the root growth and gravity response were significantly inhibited with increasing IL concentration. [C6mim]Br treatment affected the amount and distribution pattern of amyloplasts in root cap compared with controls. The auxin distribution marked withDR5rev::VENUSwas altered in IL-treated seedlings. The signal intensity and gene expression of auxin efflux carriers PIN2 and PIN3 were obviously decreased by IL stress. Moreover, as consequences in response to gravity stimulus, the asymmetric DR5 signals in control root apex were impaired by IL treatment. The predominant PIN2 signals along the lower flank of root and PIN3 polarization in columella cells were also significantly reduced in seedlings exposed to IL. Our results suggest that the ionic liquid [C6mim]Br affects the amount and distribution of amyloplasts and disturbs the deployment of PIN2 and PIN3, thus impairing auxin flows in response to gravity stimulus and causing deficient root gravitropism inArabidopsisseedlings.