mcr-1 identified in Avian Pathogenic Escherichia coli (APEC).
mcr-1 identified in Avian Pathogenic Escherichia coli (APEC).
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DOI：
10.1371/journal.pone.0172997
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发表时间：
2017
期刊：
影响因子：
3.7
通讯作者：
Logue CM
中科院分区：
文献类型：
--
作者：
Lima Barbieri N;Nielsen DW;Wannemuehler Y;Cavender T;Hussein A;Yan SG;Nolan LK;Logue CM
与粘菌素相关的抗菌素耐药性已成为全球范围内的一个重大问题，威胁着用于治疗人类疾病的最重要的抗菌素之一的使用。在此，我们检查了从美国和国际上患有大肠杆菌病的家禽中分离的禽致病性大肠杆菌（APEC）的集合（n = 980），以确定是否存在mcr-1和mcr-2（已知编码粘菌素耐药性的基因）。分析中包括一组额外的禽粪便E。大肠杆菌（AFEC）（n = 220）从健康的鸟类进行比较分析。在从中国和埃及的患病生产禽中回收的总共12个分离株中检测到mcr-1基因。在健康粪便分离株中未检测到mcr基因。使用专门设计的引物对阳性分离株的完整mcr-1基因进行测序，并与NCBI中目前描述的序列进行比较。还评估了mcr-1阳性分离株的表型粘菌素耐药性和超广谱β内酰胺表型和基因型。本研究已在至少可追溯至2010年的APEC分离株中鉴定出mcr-1，并表明在将粘菌素应用于动物卫生的国家，畜牧业实践可能导致对人类食物链的潜在耐药性来源。
Antimicrobial resistance associated with colistin has emerged as a significant concern worldwide threatening the use of one of the most important antimicrobials for treating human disease. Here, we examined a collection (n = 980) of Avian Pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis from the US and internationally for the presence of mcr-1 and mcr-2, genes known to encode colistin resistance. Included in the analysis was an additional set of avian fecal E. coli (AFEC) (n = 220) isolates from healthy birds for comparative analysis. The mcr-1 gene was detected in a total of 12 isolates recovered from diseased production birds from China and Egypt. No mcr genes were detected in the healthy fecal isolates. The full mcr-1 gene from positive isolates was sequenced using specifically designed primers and were compared with sequences currently described in NCBI. mcr-1 positive isolates were also assessed for phenotypic colistin resistance and extended spectrum beta lactam phenotypes and genotypes. This study has identified mcr-1 in APEC isolates dating back to at least 2010 and suggests that animal husbandry practices could result in a potential source of resistance to the human food chain in countries where application of colistin in animal health is practiced.