Arabidopsis ERdj3B coordinates with ERECTA-family receptor kinases to regulate ovule development and the heat stress response
Arabidopsis ERdj3B coordinates with ERECTA-family receptor kinases to regulate ovule development and the heat stress response
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拟南芥 ERdj3B 与 ERECTA 家族受体激酶协调调节胚珠发育和热应激反应
DOI：
10.1093/plcell/koac226
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发表时间：
2022-08-04
期刊：
影响因子：
11.6
通讯作者：
Chen, Li-Qun
中科院分区：
文献类型：
--
作者：
Leng, Ya-Jun;Yao, Ya-Sen;Chen, Li-Qun
拟南芥SDF 2-ERdj 3B-BiP伴侣蛋白复合物控制ERECTA家族受体激酶的转位，是胚珠珠被发育和热应激反应所必需的。内质网定位的DnaJ家族3B（ERdj 3B）是基质细胞衍生因子2（SDF 2）-ERdj 3B结合免疫球蛋白（BiP）伴侣蛋白复合物的一个组成部分，其功能是蛋白质折叠、转位，质量符合我们发现，ERdj 3B突变影响珠被发育的Ler生态型，但不是在Col-0生态型的拟南芥（拟南芥）。基于图位的克隆鉴定了ERECTA（ER）基因作为ERdj 3B的天然修饰物。ERdj 3B和ER的双突变导致热应激下内珠被的主要缺陷。额外的ER旁腺ERECTA-LIKE 1（ERL 1）或ERL 2突变为erdj 3b er双突变体加剧了缺陷的珠被表型。ER和SDF 2（SDF 2-ERdj 3B-BiP复合物的另一个组分）的双重突变导致内珠被中的类似缺陷。此外，ER，ERL 1和ERL 2的蛋白丰度和质膜分配在erdj 3b植物中显着降低，表明SDF 2-ERdj 3B-BiP伴侣复合物可能控制ERECTA家族蛋白从内质网到质膜的易位。我们的研究结果表明，SDF 2-ERdj 3B-BiP复合物在胚珠发育和热应激反应中与ERECTA家族受体激酶协调发挥作用。
The Arabidopsis SDF2-ERdj3B-BiP chaperone complex controls the translocation of ERECTA-family receptor kinases and is required for ovule integument development and the heat stress response.The endoplasmic reticulum-localized DnaJ family 3B (ERdj3B), is a component of the stromal cell-derived factor 2 (SDF2)-ERdj3B-binding immunoglobulin protein (BiP) chaperone complex, which functions in protein folding, translocation, and quality control. We found that ERdj3B mutations affected integument development in the Ler ecotype but not in the Col-0 ecotype of Arabidopsis (Arabidopsis thaliana). Map-based cloning identified the ERECTA (ER) gene as a natural modifier of ERdj3B. The double mutation of ERdj3B and ER caused a major defect in the inner integument under heat stress. Additional mutation of the ER paralog ERECTA-LIKE 1 (ERL1) or ERL2 to the erdj3b er double mutant exacerbated the defective integument phenotype. The double mutation of ER and SDF2, the other component of the SDF2-ERdj3B-BiP complex, resulted in similar defects in the inner integument. Furthermore, both the protein abundance and plasma membrane partitioning of ER, ERL1, and ERL2 were markedly reduced in erdj3b plants, indicating that the SDF2-ERdj3B-BiP chaperone complex might control the translocation of ERECTA-family proteins from the endoplasmic reticulum to the plasma membrane. Our results suggest that the SDF2-ERdj3B-BiP complex functions in ovule development and the heat stress response in coordination with ERECTA-family receptor kinases.